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Showing: 1 - 13 of 13 results

  1. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... Collections Empty Backbones Choosing Your Perfect Plasmid Backbone Empty backbones are plasmids containing...Tet-inducible lentiviral Gateway destination vector for gene expression Lentiviral backbones made by Addgene...Mammalian pLX304 - 3rd gen lentiviral vector lentiSAMv2 - Lentiviral sgRNA cloning backbone pLKO.1-blast - 3rd...Empty Backbones CRISPR Genome modification lentiCRISPR v2 - 3rd gen lentiviral vector for mammalian... Backbones Neomycin (G418) Mammalian, Varies Tet-pLKO-neo - Tet-inducible lentiviral shRNA expression... Tet-inducible lentiviral shRNA expression pLKO.1 - TRC cloning vector - Lentiviral shRNA expression...3rd gen lentiviral backbone for cloning and expression of new shRNA sequences Zeocin®/Bleo Mammalian lenti...

  2. Lentiviral Prep Service

    Type
    Collection
    ...Calabrese in backbone XPR_502 (P65 HSF), Set A Human CRISPRa sgRNA activation library in backbone XPR_502 ...blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang Cas9 and Accessories for Activating...available in lentiviral format. Viral...Brunello in lentiGuide-Puro Human sgRNA library in backbone lentiGuide-Puro targeting 19,114 genes and containing...sgRNAs along with 1000 non-targeting controls. This backbone does not contain SpCas9. 76,441 unique sgRNAs ...library in lentiCRISPRv2 Human sgRNA library in backbone lentiCRISPRv2 targeting 19,114 genes and containing...sgRNAs along with 1000 non-targeting controls. This backbone contains SpCas9. SpCas9 and 76,441 unique sgRNAs...

  3. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Mammalian/Lentiviral BbsI none S. pyogenes Puro, TagBFP Yusa lentiGuide-Puro 52963 Mammalian/Lentiviral BsmBI... Puro Zhang lenti sgRNA(MS2)_zeo backbone 61427 Mammalian/Lentiviral BsmBI none S. pyogenes Zhang pL-CRISPR.EFS.GFP...Mammalian/Lentiviral BfuAI none S. pyogenes Puro Wolfe pL-CRISPR.EFS.tRFP 57819 Mammalian/Lentiviral BsmBI...Mammalian/Lentiviral BsmBI none S. pyogenes ccdB Wei pLKO5.sgRNA.EFS.PAC 57825 Mammalian/Lentiviral BsmBI ...Mammalian/Lentiviral BsmBI yes, cut S. pyogenes Puro Ebert pL-CRISPR.SFFV.PAC 57829 Mammalian/Lentiviral BsmBI...62348 Mammalian/Lentiviral BbsI none S. pyogenes Puro Ochiai pSB700 64046 Mammalian/Lentiviral none S. pyogenes...57822 Mammalian/Lentiviral BsmBI none S. pyogenes EGFP Ebert pSECC 60820 Mammalian/Lentiviral BsmBI yes, cut...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ...important in disease. Empty Backbones This table is a collection of empty backbone plasmids into which you...with Nano-lanterns Collection Highlights Empty Backbones Expression Constructs Reporter Constructs Luciferase...regulatory elements on gene expression. Empty backbones for inserting regulatory elements before or after...promoter/enhancer regions using Gateway cloning. Lentiviral. Jorge Ferrer 87067 pcDNA3.1-ccdB-Nanoluc NanoLuc...N-terminal Nanoluc fusions using Gateway cloning. Lentiviral. Mikko Taipale 12563 PCD FL0X Firefly Insertion...Firefly luciferase fusions using Gateway cloning. Lentiviral. Mikko Taipale 87077 pLenti6.2-Firefly-ccdB Firefly...Firefly luciferase fusions using Gateway cloning. Lentiviral. Mikko Taipale 87789 pPTluc Firefly Insertion...

  5. Mammalian RNAi Tools

    Type
    Collection
    ...Most plasmids are in a lentiviral backbone, with some retroviral and AAV backbone options. Plasmids for...Addgene; browse by constitutive lentiviral shRNA, cre-regulated lentiviral shRNA, and more. Plasmid...shRNAs are often delivered using viral vectors. Lentiviral vectors are a preferred method for long-term ...vector protocol Other Addgene viral protocols Lentiviral packaging plasmids Web Resources Trono Lab Lentivectors...

  6. Zhang Lab CRISPR Page

    Type
    Collection
    ...2A Hygro resistance marker 61427 : lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop...60231 : sgRNA cloning backbone with Cre recombinase-2A-EGFP-KASH Detailed backbone cloning information:...#61425 - #61427 are intended for lentivirus production : 61425 : lentiviral vector encoding dCAS9-VP64 with... top GeCKO Screening Library GeCKO are pooled lentiviral CRISPR libraries (both human and mouse) for performing...helper complex with 2A GFP 61424 : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop - Contains...with 2A Blast resistance marker 61426 : lentiviral vector encoding the MS2-P65-HSF1 activator helper complex...luciferase-2A-Cre recombinase 60226 : sgRNA cloning backbone with luciferase-2A-Cre recombinase Vectors for...

  7. Cre-lox system

    Type
    Collection
    ...12238 pLOX-CW-CRE Cre CMV Lentiviral Trono 12265 pHR-CMV-nlsCRE Cre CMV Lentiviral Trono 12493 p259 pCMV-...coexpression CMV Lentiviral Sadelain 30205 Cre-IRES-PuroR Cre and PuroR coexpression EF-1 alpha Lentiviral Kotton...-cre mCherry and Cre Lentiviral Luikart 66691 FUG-T2A-cre GFP and Cre Lentiviral Luikart 66692 pRubiC-...-iCre copGFP and iCre CB Lentiviral Oka 72257 pCDH-CB-iCre iCre CB Lentiviral Oka 73350 pAdx-CMV-iCre-...components, PhyBdeltaCreC and PIF6CreN, in lentiviral vector Cbh Lentiviral Eisenhoffer 132551 AAV-U6-gRNA-TnT-Cre...Cre, Puro resistance and miRNA expression PGK Lentiviral Jacks 17736 pOG231 NLS-Cre CMV Mammalian Wahl... LV-Cre pLKO.1 Cre and shRNA coexpression CMV Lentiviral Fuchs 26646 pCAG-Cre-IRES2-GFP Cre and GFP coexpression...

  8. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Fluorescent Protein Guide Empty Backbones Fluorescent Protein Guide: Empty Backbones More Fluorescent Protein... mRFP Tsoulfas Lab Lentiviral Plasmids - Fluorescently tag your gene for lentiviral expression Verkhusha...Addgene has assembled a collection of empty plasmid backbones with different fluorescent tags for you to create...generated with the help of Erik Snapp . Empty Backbones for Fluorescent Protein Fusions (Organized by ...Prone to dimerization pLV-Azurite - Mammalian Lentiviral expression pB18cmBFPAzurite - Bacterial Expression...pCAG-GFP - Mammalian Expression pLV-eGFP - Mammalian Lentiviral Expression Ac5-STABLE1-neo - Insect Expression...

  9. Tetracycline Inducible Expression

    Type
    Collection
    ...Resources: Browse Tetracycline Plasmids Tet-regulated Lentiviral RNAi Vectors Curated Plasmid Collections Background...tetracycline-free. Tetracycline-inducible Empty Backbones Find a construct that will allow you to insert...On or Off PI 21916 Tet-pLKO-neo 3rd generation lentiviral plasmid for inducible expression of shRNA; neomycin... selection TetR On Wiederschain 41393 pCW57.1 Lentiviral vector for inducible expression for Gateway cloning...insert options rtTA On Root 11651 pLVUT-tTR-KRAB Lentiviral vector for inducible expression of transgene ...tTA or rtTA None On Vogelstein 25735 pSLIK-Neo Lentiviral vector for inducible expression of a miR-shRNA...third-generation rtTA On Fraser 44012 pInducer20 Lentiviral vector for shRNA expression; see article for ...

  10. Adenovirus Plasmids

    Type
    Collection
    ...Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...shuttle plasmid and an adenoviral (also called backbone) plasmid, are recombined into a DNA molecule that...cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses , adenoviruses do not ...

  11. CRISPR References and Information

    Type
    Collection
    ...CRISPOR batch (Link opens in a new window) for lentiviral screens. sgRNA Scorer 2.0 (Link opens in a new...Nematode: Cas9 and gRNA use Cas9 (pIK86) ; gRNA empty backbone (pDR274) PDF, 89 KB Liu Fly: gRNA cloning pAc-...sequencing Cas9: pMLM3613 dsDNA donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED PDF, 597...

  12. CRISPR Guide

    Type
    Collection
    ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...been created in AAV backbones for in vivo experiments and in a retroviral backbone for delivery to cells...disrupt Cas9’s interactions with DNA phosphate backbone HypaCas9 - increase Cas9 proofreading and discrimination...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...

  13. Plasmid Collections

    Type
    Collection
    ...Collection Bacterial Expression Systems Empty Backbones Genetic Code Expansion Immunology Mammalian RNAi... are a set of plasmids all built with the same backbone and only differing in a small region. Click here...Luciferase Optogenetics Chemogenetics Viral Plasmids Lentivirus Adeno-associated Virus (AAV) γ-Retrovirus Adenovirus...
Showing: 1 - 13 of 13 results