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We narrowed to 9 results for: lentiviral particles

Showing: 1 - 9 of 9 results

  1. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...collection: HIV-based lentiviral particles Murine leukemia-based retroviral particles Vesicular stomatitis...2A-MCS-Hygro - Lentiviral vector expressing destabilized EGFP. Lenti-luciferase-P2A-Neo - Lentiviral reporter...- Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein Nemazee lab...) are packaged as replication-defective virus particles with the SARS-CoV-2 spike (S) glycoprotein on...on the envelope surface. Pseudotyped virus particles can then be used to infect permissive cells that ...infective potential. The following types of viral particles have been pseudotyped with the SARS-CoV-2 Spike...stomatitis virus particles See Addgene's Viral Plasmids and Resources page for more information. Plasmids Expressing...

  2. Viral Production

    Type
    Collection
    ...vectors consist of full particles (white arrowhead) relative to empty particles (black arrowhead). Scale...for in our reported titers. Lentiviral vectors are titered using a lentiviral droplet digital PCR (ddPCR...AAV vectors collection. AAV Data Hub Lentivirus Production Lentiviral preparations are produced in 293T ...performed on lentiviral preparations that have been stored at -80 °C and thawed. Lentivirus titration is...produced your viral vector lot. At Addgene, AAV particles are titered by droplet digital PCR (ddPCR) using...of empty to full (i.e., genome containing) AAV particles within representative vector preparations was ...microscopy after negative staining. Empty vector particles can be identified after negative staining and ...

  3. Lentivirus Plasmids

    Type
    Collection
    ...Blog: Tips for Titering Your Lentiviral Preps Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids...researcher safety. Read our Lentiviral Guide for more information about: Lentiviral components 2nd versus 3rd...Glossary of commonly used lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature...Reiser Return to Top Popular Lentiviral Transfer Plasmids The lentiviral genome (i.e., the genetic material...difficult to efficiently produce viral particles). This table lists lentiviral transfer plasmids that can be used...generation lentiviral systems Viral production Frequently asked questions about lentivirus use Glossary...enclosed in the viral particle) is delivered to a target cell upon infection. When lentivirus is used for research...

  4. Lentiviral Prep Service

    Type
    Collection
    ...CellTag Barcode Library Version 1 Ready-to-use lentiviral particles carrying version 1 of the CellTag barcoding...CellTag Barcode Library Version 2 Ready-to-use lentiviral particles carrying version 2 of the CellTag barcoding...CellTag Barcode Library Version 3 Ready-to-use lentiviral particles carrying version 3 of the CellTag barcoding...from the inventory below. Lentiviruses are distributed as purified viral particles in solution that are suitable...available in lentiviral format. Viral...and blasticidin resistance from EFS promoter. Lentiviral backbone. Zhang Cas9 and Accessories for Activating... CMV GFP Hygro (656-4) GFP Hygromycin 3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro Campeau...

  5. Viral Prep Service for Plasmids and Pooled Libraries

    Type
    Collection
    ...In-Stock Preps We offer commonly used AAV and lentiviral vectors as in-stock preps. These high-titer preps...distributed as 100 µL solutions of purified viral particles that are suitable for in vivo use. Order a high-quality... libraries. Viral preparation options include lentivirus and AAV. Viral...high-quality and ready-to-ship AAV or lentivirus prep for your next experiment today! Browse AAV | Browse...

  6. Plan Your Experiment

    Type
    Collection
    ...produce the viral particles. Then you transduce your cells using the viral particles before you continue...production, see our viral vector guides . Lentiviral vectors Lentiviral vectors are a popular option for producing...types like primary cells. For CRISPR delivery, lentiviral and adeno-associated virus (AAV) vectors are ...CRISPR components) integrates into the genome. Lentiviral vectors are a good option for difficult-to-transfect...we don’t discuss CRISPR screens in this guide, lentiviral vectors are a common choice for conducting genome-wide...CRISPR . Browse Addgene's collection of in-stock lentiviral preps . AAV vectors AAV vectors offer some advantages...advantages for viral vector delivery. Both lentiviral and AAV vectors are safe to use in the lab, but ...

  7. Adenovirus Plasmids

    Type
    Collection
    ...Additional Resources: Adenovirus Guide Virus Biosafety Lentiviral Plasmids AAV Plasmids Viral Vectors 101 eBook...mammalian packaging cell lines to generate adenovirus particles. Read our Adenoviral Guide for more information...cell types with almost 100% efficiency. Unlike lentiviruses or other retroviruses , adenoviruses do not ...

  8. Neurodegeneration Research Collection

    Type
    Collection
    ... Proc Natl Acad Sci U S A. 2023 Aug 22. Use a lentiviral FRET-based reporter to study tau seeding in mammalian...AAV viral preps for systemic delivery of viral particles that will target the central nervous system, peripheral...

  9. CRISPR Guide

    Type
    Collection
    ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled...capabilities but are small enough to be packaged in AAV particles. Cas13 fusions have also been used in in vivo ...
Showing: 1 - 9 of 9 results