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  1. Qi Lab CRISPR Page

    Type
    Collection
    ...targeting endogenous CXCR4 gene 46920 pTDH3-dCas9 Yeast CEN/ARS vector (Leu2) that contains dCas9 fused ...controlled by TDH3 promoter 46921 pTDH3-dCas9-Mxi1 Yeast CEN/ARS vector (Leu2) that contains dCas9 fused ...integrated into HEK293 genome 46922 pSNR52-sgTEF1 Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled...targeting endogenous TEF1 promoter 46923 pSNR52-sgTET Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled... pU6-sgGAL4-1 Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter... pU6-sgGAL4-4 Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter...

  2. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...TARDBP GAL ALS Aaron Gitler 27465 pDuet TDP-43 Q331K TARDBP GAL ALS Aaron Gitler 27466 pRS426 Gal TDP43...TDP43 TARDBP GAL ALS Aaron Gitler 27467 pRS426 Gal TDP43 GFP TARDBP GFP GAL ALS Aaron Gitler 27468 pRS303... pAG303-Gal-PR50 C9orf72 FLAG, Myc GAL1 ALS Aaron Gitler 84906 pAG303-Gal-PA50 C9orf72 FLAG, Myc GAL1 ...84907 pAG303-Gal-GA50 C9orf72 FLAG, Myc GAL1 ALS Aaron Gitler 84908 pAG303-Gal-GR100 C9orf72 GAL1 ALS Aaron...181709 pAG416-GAL-TDP-43 TARDBP GAL1 ALS Alejandro Chavez 181710 pAG416-GAL-hnRNPA1 HNRNPA1 GAL1 ALS Alejandro...Chavez 181711 pAG416-GAL-FUS FUS GAL1 ALS Alejandro Chavez 181716 pAG416-GAL-FUS-G156E FUS GAL1 ALS Alejandro...181717 pAG416-GAL-FUS-P431L FUS GAL1 ALS Alejandro Chavez 181718 pAG416-GAL-FUS-R521C FUS GAL1 ALS Alejandro...

  3. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ... expression URA3 Yeast pXP518 - Yeast expression vector pPS808 - Yeast expression ...) TRP1 Yeast pXP216 - Yeast expression vector (PGK1 promoter, 2μ) pXP316 - Yeast expression...LEU2 Yeast pXP122 - Yeast expression vector (PGK1 promoter, CEN/ARS) pXP222 - Yeast expression...vectors) HIS3 Yeast pXP220 - Yeast expression vector (PGK1 promoter, 2μ) pAG303GAL-ccdB - Yeast...Set of yeast expression vectors with various markers, promoters, etc pRS420 - Yeast expression...purification or localization experiments Yeast GAL4, PGK, ADH1, ADE2, TRP1 Gateway destination ... Xenopus pAG423GPD-ccdB-HA - C-terminal 3xHA tag for yeast expression Myc Epitope...

  4. Cre-lox system

    Type
    Collection
    ...optimized for yeast Gal1 Yeast Sandmeyer 26853 pBF3060 Cre codon optimized for yeast Gal1 Yeast Sandmeyer ...mutant TEF2 Yeast Piper 60931 pPL5608_TEF1*-Cre_TRP1 Cre expressed at low levels mutant TEF1 Yeast Piper 60932...mutant TEF1 Yeast Piper 60933 pPL5628_TEF1*-Cre_MET15 Cre expressed at low levels mutant TEF3 Yeast Piper 61391...TDH3 Yeast van Leeuwen 64770 pSS146 Cre fused to the human Estrogen Binding Domain (EBD) Tdh4 Yeast van...pSH62-EBD Cre-EBD - Estradiol-dependent control Gal1 Yeast Gartenberg 50797 pUAS-Cre Cre UAS/Hsp70 Mammalian...recombinase CAG AAV Bruchas 78398 pNXRVa-HACre Cre CMV Yeast Tomita 82587 pME-ERT2-Cre-ERT2 Tamoxifen-inducible...Capecchi 137862 pCAGGS-mTagBFP2-T2A-iCreERT2 (PAGSA) iCre-ERT2 (PAGSA N-terminus) - Tamoxifen inducible CAG Mammalian...

  5. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Basta Kim pMZ283 52224 Yeast CspCI none S. pyogenes URA4 Zaratiegui pJZC588 62315 Yeast none S. pyogenes URA3...gRNA_handle_RPR1t 62966 Yeast HindIII none S. pyogenes Puro Lu pML104 67638 Yeast BclI-SwaI yes, cut S. ...Wyrick pML107 67639 Yeast BclI-SwaI yes, cut S. pyogenes LEU2 Wyrick pT040 67640 Yeast BclI-SwaI none S....Jackson pMZ376 74213 Yeast rrk1 yes, cut S. pyogenes KanMX Zaratiegui pMZ377 74214 Yeast rrk1 yes, cut S. ...enhanced) S. pyogenes EGFP Zou pRS416gT-GalL-Cas9 79903 Yeast RPR1(TetO) yes, cut S. pyogenes URA3 Davis... Mammalian Bacteria Drosophila Plant C. elegans Yeast Zebrafish Xenopus CRISPR Resources CRISPR Guide ...Mammalian, Lentiviral, AAV, Bacteria, Drosophila, Yeast, Plant, Xenopus, Worm, or Other. Whether the plasmid...

  6. TALEN Plasmids and Kits

    Type
    Collection
    ...TALORs (TAL Orthongal Repressors) that can be used to custom repress gene expression in yeast. TALORs consist...-BB contains the GAL1 promoter, placing TALORs built into this vector under galactose-inducible expression...Mendenhall The TALE plasmid vectors described on this page are designed to target and edit the epigenome of...

  7. CRISPR Guide

    Type
    Collection
    ...libraries are also available, such as those for yeast or drosophila . Although CRISPR has been less widely...SpCas9 variant is identified by in vivo screening in yeast. Nature Biotechnology , 36 (3), 265–271. PMID: 29431739...plasmids and resources at Addgene Blog CRISPR topic page How to design your gRNA for CRISPR genome editing... Gainetdinov, I., Yoon, Y., Song, C., Cao, Y., Gallant, J., Xue, W., Rivera-Pérez, J. A., & Sontheimer... Manchado, E., Concepcion, C. P., Bonetti, C., Vidigal, J. A., Han, Y., Ogrodowski, P., Crippa, A., Rekhtman...
Showing: 1 - 7 of 7 results