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  1. Lentivirus Plasmids

    Type
    Collection
    ...along with additional packaging components Trono 63586 psPAX2-D64V 2nd Packaging For making Integrase ... with a plasmid encoding Rev Trono 12260 psPAX2 2nd Packaging Contains Gag, Pol, Rev, and Tat; can be ...22500 pNHP 2nd Packaging Contains gag, pol, tat, rev Kasparov 1732 pCPRDEnv 2nd Packaging part of the FELIX...strain). Use with packaging construct encoding Tat. Reiser 17531 pCD/NL-BH*DDD 2nd Packaging Contains Gag/...Addgene lentiviral packaging, envelope, and transfer plasmids Viral...Lentiviral Pooled Libraries All Viral Plasmids Packaging Plasmids Transfer Plasmids Lentiviruses, a subset...lentiviral terms Popular Lentiviral Envelope and Packaging Plasmids One safety feature of lentivirus used...

  2. CRISPR References and Information

    Type
    Collection
    ...amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro packaging plasmids... gRNA design and cloning gRNA cloning vector Retroviral vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) ...Zhang sgRNA cloning lentiCRISPR v2 packaging plasmids: pVSVg , psPAX2 ; positive control: CMV-EGFP PDF ...protocol Church gRNA design and cloning gRNA cloning vector PDF 115.2 KB Church gRNA design and cloning for...human pluripotent stem cells pCas9_GFP ; gRNA empty vector Link O'Connor-Giles Fly: gRNA and ssODNs design...pX330) Link Parrott NEBuilder Assembly of CRISPR vectors using ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H...plasmids: pVSVg , psPAX2 positive control: CMV-EGFP Kits are also available (mouse or human libraries) PDF...

  3. Viral Production

    Type
    Collection
    ... Viral Vector Packaging Service Viral Production Viral Production ... with viral vector manufacturing facilities, such as the University of Pennsylvania Vector Core (Link ...Pennsylvania Vector Core. In general, titering is performed by the facility that produced the viral vector lot....Empty vector particles can be identified after negative staining and appear darker than full vector particles...Electron micrograph of AAV vector preparation shows that the vast majority of the vectors consist of full particles... 293T cells using the 2nd generation psPAX2 and pMD2.G packaging system developed by the Trono lab . Cell...to determine which facility produced your viral vector lot. At Addgene, AAV particles are titered by droplet...
Showing: 1 - 3 of 3 results