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We narrowed to 3 results for: px330 vector expressing cas9

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Showing: 1 - 3 of 3 results

  1. CRISPR Plasmids - Tagging

    Type
    Collection
    ...Tagging Plasmids: pX330S-2-PITCh - expresses Cas9 & PITCh-gRNA pX330A-FBL/PITCh - expresses Cas9, PITCh-gRNA...components: An all-in-one CRISPR/Cas9 vector based on the Zhang lab's PX330 , simultaneously targeting the...gene-specific CRISPR and donor vectors as well as pX330S-2-PITCh, expressing a generic gRNA targeting the...targeting vector assembly, and the remaining 5 plasmids include Cre and FLP expression vectors, a general... one vector from the Doyon lab, eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Addgene #79888) , which expresses an untagged...plasmid-system consists of two components: A vector containing Cas9 and a validated gRNA, based on the Zhang... that uses PCR to generate both an expression cassette for the Cas9-programming sgRNA and HR donors for...

  2. CRISPR References and Information

    Type
    Collection
    ...CRISPR RNA array: Cas9 (pX260) or Cas9 D10A (pX334) ; tracrRNA: Cas9 (pX330) or Cas9 D10A (pX335) PDF,...sites for S. pyogenes Cas9, S. aureus Cas9, S. thermophilus Cas9, N. meningitidis Cas9, or Cas12a from your...ssDNA oligos p201G Cas9 ; p201B Cas9 ; p201H Cas9 ; p201N Cas9 ; pUC gRNA Shuttle PDF, 500 KB Sabatini and... gRNA cloning vector PDF, 118 KB Church gRNA design and cloning for Cas9 orthologs Cas9 plasmids PDF, ...gRNA core ; Cas9 ; optimized Cas9 PDF, 66.8 KB Fujii gRNA design and cloning gRNA cloning vector Retroviral...Mammalian Cell Lines pSpCas9(BB) (pX330) PDF, 330 KB Parrott NEBuilder Assembly of CRISPR vectors using ssDNA ...library amplification 1 vector system: lentiCRISPR v2 2 vector system: lentiCas9-Blast and lentiGuide-Puro...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    .... The dual vector system uses S. pyogenes Cas9 (SpCas9), using one vector to express SpCas9, and another...constitutively Cas9-expressing mouse. Described here are AAV vectors that can be combined with Cas9 in a wide...information: CRISPR-Cas9 mouse toolbox protocol 637.8 KB CRISPR-Cas9 Cre expression vectors for cancer modeling... (20bp). CRISPR-Cas9 Cre expression vectors for genome editing in the brain Using Cas9 mice Platt et al...constitutively expressing Cas9 knockin mice (Platt et al ., Cell 2014). In these mice the CRISPR-Cas9 system ...formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast and...2013). SpCas9 Cas9: The PX551 plasmid (AAV-SpCas9, #60957) contains a single cassette expressing human ...
Showing: 1 - 3 of 3 results