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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7 results for: stem loop

Showing: 1 - 7 of 7 results

  1. AAV Molecular Tools

    Type
    Collection
    ...tet-off transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Gradinaru 99121 ...tet-off transactivator (tTA) with a positive feedback loop for amplified tTA expression. 1 Gradinaru 117383...tetracycline (tet)-inducible expression systems . ID Name Expression System Activity Serotype PI 99118 pAAV-CAG-tTA... in the CRISPR-Cas9 system for targeted genome editing. ID Name Expression System Activity Serotype PI...can be used for viral TRAP). ID Name Expression System Activity Serotype PI 98747 pAAV-FLEX-EGFPL10a EF1a-driven...neuronal connectivity labeling. ID Name Expression System Activity Serotype PI 51509 AAV phSyn1(S)-FLEX-tdTomato-T2A-SypEGFP-WPRE...can be used for cell ablation. ID Name Expression System Activity Serotype PI 124364 pAAV-FLEX-DTR-GFP CBA-driven...

  2. Malate Dehydrogenase CUREs Community Collection

    Type
    Collection
    ... (specific mutation, "active site", "active site loop", or "subunit interface") ID Plasmid Description...Collaboration Results in Student Learning Gains and Positive STEM Attitudes in CUREs. Callahan KP et al. CBE Life ...

  3. Zhang Lab CRISPR Page

    Type
    Collection
    ... : sgRNA cloning backbone with MS2 loops at tetraloop and stemloop - Contains BbsI sites for insertion...lentiviral sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains... formats: 1 vector system - lentiCRISPR - sgRNA and SpCas9 together 2 vector system - lentiCas9-Blast ...incorporating two MS2 RNA aptamers at the tetraloop and stemloop 2 The MS2-P65-HSF1 activation helper protein... P alindromic R epeats) is a microbial nuclease system involved in defense against invading phages and.... We have harnessed the type II CRISPR nuclease system to facilitate genome editing in mammalian cells...Protocol 237.2 KB SpCas9 Plasmids The CRISPR/Cas system can be implemented in mammalian cells by co-expressing...

  4. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker pLenti... 2xFLAG tag in pCS for a variety of systems, including Xenopus HA Epitope tag...ultraID in mammalian cells with the Tet-On or Tet-Off system Return to top Selectable Markers Regardless of ...

  5. CRISPR Guide

    Type
    Collection
    ...Mice with Haploid Embryonic Stem Cells Carrying a Guide RNA Library. Cell Stem Cell . 17 (2), 221–232. PMID... Taylor, D. W. (2023). Structural snapshots of R-loop formation by a type I-C CRISPR Cascade. Molecular...enough to cause a DSB. The nickase system is utilized in many CRISPR systems to achieve specific genetic edits...Cas9 (CRISPR Type II) systems, while AcrV inhibits Cas12a (CRISPR Type V) systems. There are rare cases...The two main classes of CAST systems are type I and type V-K. Type I systems use a type I Cas effector, ...), while type V-K systems use a type V-K Cas effector, Cas12k. No matter the system, the Cas effectors...-vector systems, in which Cas9 is included in the gRNA-containing plasmid, or 2-vector systems, in which...

  6. Immunology Research Plasmids and Resources

    Type
    Collection
    ...calcineurin B homologous protein 2 - CHUK conserved helix-loop-helix ubiquitous kinase IKBKA, IKK-alpha, IKK1, ...infection depends on two systems that work in concert: the innate and adaptive immune systems. Upon infection,... Lentiviral Expression (MuLE) System Kit : A modular and flexible system that can be used to construct...infection, the immune system triggers several processes to activate cells to recognize and get rid of infectious...following exposure to a pathogen. The innate immune system is not specific to any particular pathogen. It ...Cytokines are molecules that enable cells of the immune system to communicate with each other across long distances...

  7. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Halo CBA ALS Marvin Bentley 134901 pUC19-hAPOE-R-loop APOE T7 Alzheimer's Andrew Deans 137187 pAAV FLEX... Multisystem proteinopathy, ALS Nicolas Fawzi 98667 hnRNPA2_LC_WT_S329C HNRNPA2B1 His T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139121 hnRNPA2_LC_NtoS HNRNPA2B1 T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139123 hnRNPA2_LC_12E HNRNPA2B1 T7 Multisystem proteinopathy...HNRNPA2B1 T7 Multisystem proteinopathy, ALS Nicolas Fawzi 139125 hnRNPF_PLD_StoA HNRNPA2B1 T7 Multisystem proteinopathy... T7 Multisystem proteinopathy, ALS Nicolas Fawzi 157865 hnRNPA2_RRM1 HNRNPA2B1 His T7 Multisystem proteinopathy... Dementia Joan Massague 58260 pBabe-Neuroserpin oloop SERPINI1 Dementia Joan Massague 58261 pBabe-FLAG-Neuroserpin...
Showing: 1 - 7 of 7 results