Genetic Code Expansion
Type
Collection
...aminoacyl-tRNA synthetase (aaRS) enzyme loads a specific amino acid to its tRNA. The charged tRNA is then...specifically load the new tRNA with the ncAA. Together, the engineered tRNA and the aaRS enzyme form an...enzyme should not recognize endogenous tRNAs, and the orthogonal tRNA should not be an efficient substrate...click amino acids, tRNA M15 Mammalian TAG Irene Coin 113644 pRF0G-Tyr tyrosyl-tRNA synthetase M. jannaschii...archaeal MjTyrRS/tRNA pair instead. For directed evolution of the liberated EcTyrRS/tRNA pair to incorporate... endogenous EcTyrRS/tRNA pair (tyrS gene knocked out, tyrTV gene replaced by tRNA variant that suppress...