Genetic Code Expansion
Type
Collection
...aminoacyl-tRNA synthetase (aaRS) enzyme loads a specific amino acid to its tRNA. The charged tRNA is then...specifically load the new tRNA with the ncAA. Together, the engineered tRNA and the aaRS enzyme form an...enzyme should not recognize endogenous tRNAs, and the orthogonal tRNA should not be an efficient substrate...click amino acids, tRNA M15 Mammalian TAG Irene Coin 113644 pRF0G-Tyr tyrosyl-tRNA synthetase M. jannaschii...pyrrolysine (Pyl) tRNA synthetase M. mazei Mammalian TAG Ivana Nikić-Spiegel 182881 Mut1-RS tyrosyl-tRNA synthetase...pEVOL_PylRS(AF)-tRNA PylRS (Y306A, Y384F) M. mazei Mammalian TAG Edward Lemke 223512 pEVOL_PylRS(WT)-tRNA PylRS ...Expresses yeast TrpRS/tRNA pair instead. For directed evolution of the liberated EcTrpRS/tRNA pair to incorporate...