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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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In Category

We narrowed to 5 results for: trna

Showing: 1 - 5 of 5 results

  1. Genetic Code Expansion

    Type
    Collection
    ...aminoacyl-tRNA synthetase (aaRS) loads its cognate tRNA with a specific amino acid. Then, the tRNA is pulled.... To expand the genetic code, modified tRNAs, codons, and tRNA synthetases are introduced into the cell... below: A plasmid expressing the tRNA and its cognate aminoacyl-tRNA-synthetase (aaRS) that has been evolved...have also been utilized. A tRNA that recognizes this codon. An aminoacyl-tRNA synthetase to load the new...new amino acid onto the tRNA. The tRNA and synthetase are called an orthogonal set, because they should...orthogonal tRNA, and not endogenous ones. Endogenous synthetases cannot aminoacylate the orthogonal tRNA. And...click amino acids, tRNA M15 Mammalian TAG Irene Coin 113644 pRF0G-Tyr tyrosyl-tRNA synthetase M. jannaschii...

  2. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...phosphoserine using SepOTSλ ( 68292 ) or serine using supD tRNA ( 68307 ) at the central position. Introducing Hi-P...phosphosite library can also be synthesized using supD tRNA ( 68307 ) to generate the phosphosites with serine...EF-Sep ( aka. pKD-SepRS-EFSep) Plasmid 34624 SepOTSα tRNA-Sep ( aka. pCAT112TAG-SepT) Plasmid 68283 SepOTSβ...

  3. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...capability with the endogenous tRNA-processing system Plant Utilizes the tRNA processing system to reduce... pCRISPRyl 70007 Other/Yarrowia lipolytica SCR1'-tRNA yes, cut S. pyogenes LEU2 Wheeldon FgH1tUTG 70183...vector. sgRNAs are expressed as polycistronic glycine tRNA-gRNA genes (PTGs) and then PTG cassettes are assembled...promoters. Bullock pCFD5 Drosophila Utilizes endogenous tRNA processing machinery to excise multiple functional...

  4. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...directed evolution of the Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine base editors, the...

  5. CRISPR Guide

    Type
    Collection
    ...directed evolution of the Escherichia coli TadA, a tRNA adenosine deaminase. Like cytosine base editors,...
Showing: 1 - 5 of 5 results