Optogenetics Guide
Type
Guide
...protein clusters through CRY2-CIB1 oligomerization 450 OPTOSTIM PHR domain of CRY2 from A. thaliana is fused.... thaliana Cryptochrome 2 and CIB1 450 CRY2olig, CRY2clust CRY2 variants that undergo homo-oligomerization...widely-used systems is the A. thaliana cryptochrome Cry2 and its binding partner cryptochrome-interacting...CIB1. Blue light induces a conformational change in Cry2 that allows CIB1 binding, which can be utilized ...interest. For example, a genetically-encoded nuclear Cry2 fusion can recruit a CIB1-fused protein to the nucleus...of interest, such as Cre recombinase, is fused to Cry2 and the other fragment to CIB1, light-stimulated...to generate two-part transcription factors, where Cry2 and CIB1 are fused to a transcriptional activation...