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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 3 results for: Folding Reporter GFP

Showing: 1 - 3 of 3 results

  1. Plan Your Experiment

    Type
    Guide
    ...is typically used for gRNA May contain reporter gene (e.g. GFP) to identify and enrich positive cells,... separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich...facilitates CRISPR/Cas9-based genome editing. Scientific Reports . 5 , 11315. PMID: 26066060 Zuris, J. A., Thompson...and inserted into plasmids containing the gRNA scaffold using standard restriction-ligation cloning. However...

  2. Optogenetics Guide

    Type
    Guide
    ...optical switches Sensors are genetically-encoded reporters of molecular signals; e.g., calcium indicators...plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk...photoswitch. Illumination causes the photoswitch to unfold, lowering the toxin's local concentration near ...

  3. CRISPR Guide

    Type
    Guide
    ...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...mapping multiprotein complexes in human cells. Cell Reports . 13 (3), 621–633. PMID: 26456817 Doench, J. G....The gRNA is a short synthetic RNA composed of a scaffold sequence necessary for Cas-binding and a user-...
Showing: 1 - 3 of 3 results