We narrowed to 1 result for: Folding Reporter GFP

...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). https://doi.org/10.1038/srep05400 PMID...mapping multiprotein complexes in human cells. Cell Reports . 13 (3), 621–633. https://doi.org/10.1016/j.celrep...The gRNA is a short synthetic RNA composed of a scaffold sequence necessary for Cas-binding and a user-...