...control expression of a chemogenetic reporter ensures that the reporter remains silent until a cell or tissue-specific...light-emitting luciferase and a light-sensing optogenetic element. When the luciferase substrate (luciferin) is ...efficiencies. Depending on the type of luciferase used, the luciferin substrate may be the native coelenterazine...in neurons LMO Luciferase Opsin Ligand Effect Outcome (in neurons) Reference LMO3 sbGLuc VChR1 CTZ Na +... activation Slaviero et al., 2024 iLMO: RLuc-NpHR Venus-Rluc8 NpHR hCTZ Cl - influx Neuronal inhibition...intracellular calcium stores. There are currently three Gq DREADDs based on human muscarinic receptors: ...is added, the luciferase enzyme generates light that activates the opsin. The opsin may excite or inhibit...

...elements. These plasmids contain a reporter gene (for example, luciferase or GFP) that offers a read-out ...information on CRISPR check out Addgene’s CRISPR guide . Reporter Plasmids - Used for studying the function of genetic...of interest could be inserted upstream of the luciferase gene to determine the level of transcription ...V A, C, or G W A or T Y C or T Amino Acids Name Three Letter Code Single Letter Code Codons (RNA) Alanine... CCG Serine Ser S UCU, UCC, UCA, UCG, AGU,AGC Threonine Thr T ACU, ACC, ACA, ACG Tryptophan Trp W UGG ...GUG Start AUG* Stop UAG (amber), UGA (opal), UAA (ochre) *AUG is the most common start codon. Alternative...