Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 5 results for: TOP-GFP reporter element

Showing: 1 - 5 of 5 results

  1. Adenovirus Guide

    Type
    Guide
    ...pAdTrack series contains an IRES-GFP construct that enables co-expression of GFP with the transgene of interest...in a new window) Adenoviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose...Common Uses Safety Resources and References Plasmid Elements Glossary Adenoviruses (AdV) are medium-sized, ... cold. The immune system recognizes adenoviral elements rapidly and can mount a strong immune response...plasmid components, see the Adenoviral Plasmid Elements table . Adenoviral Generations First-generation...throughout viral vector production. During experiments, GFP can be used to sort cells infected with rAdV vectors...genome. Instead, they remain as extrachromosomal elements within the nucleus, normally as double-stranded...

  2. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead...: 12805549 Gamma-Retroviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose...Viruses Viral Vector Elements More Viral Vectors 101 topics More Retroviral Vector topics Addgene protocols...Pseudotyping Common Uses Safety Resources Plasmid Elements Glossary Gamma-retroviruses are a subtype of retrovirus...plasmid components, see the Gamma-Retroviral Plasmid Elements table . In order to produce gamma-retroviral vectors... sorting (FACS) is used to sort cells expressing GFP and later, sorted cells are expanded into a cell ...hepatitis virus post‐transcriptional regulatory element; stimulates the expression of transgenes via increased...

  3. Lentiviral Vector Guide

    Type
    Guide
    ... type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker. Instead...871 PMID: 9306402 Lentiviral Plasmid Elements Plasmid Type Element Delivery relative to transgene Purpose...Pseudotyping Common Uses Safety Resources Plasmid Elements Glossary Lentiviruses are a type of retrovirus...plasmid components, see the Lentiviral Plasmid Elements table . In order to produce lentiviral vectors... sorting (FACS) is used to sort cells expressing GFP and later expanded into a cell line. More information...Tat binds. TAR in cis Trans-activating response element; found in the R region and acts as the binding ...hepatitis virus post‐transcriptional regulatory element; stimulates the expression of transgenes via increased...

  4. Molecular Biology Reference

    Type
    Guide
    ...plasmid. Below are some common plasmid elements: Plasmid Element Description Origin of Replication ( ori... CRISPR guide . Reporter Plasmids - Used for studying the function of genetic elements. These plasmids...plasmids contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity of ...Biology Basics Origins of Molecular Genetics Plasmid Elements Working with Plasmids Introduction Types of Plasmids...easily study and manipulate genes and other genetic elements using specifically engineered plasmids, commonly...guide on molecular cloning techniques . Plasmid Elements Plasmids used by scientists today come in many...unique restriction enzyme recognition site. These elements allow for the propagation of the plasmid within...

  5. CRISPR Guide

    Type
    Guide
    ...and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a, it will release... using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the reaction...have evolved in phages and other mobile genetic elements to overcome the CRISPR systems that bacteria and...integrating segments of DNA flanked by specific repeat elements into the genome. CRISPR-associated transposases...fluorescent marker like green fluorescent protein (GFP), creating a customizable DNA or RNA label for fluorescence... target sequence, it promiscuously cleaves the reporter as well, and a quantifiable signal is produced...all-in-one CRISPR/Cas9 vector system. Scientific Reports , 4 (1). PMID: 24954249 Shechner, D. M., Hacisuleyman...
Showing: 1 - 5 of 5 results