...your own research. CRISPR Basics In this section, we will introduce the basic mechanisms of CRISPR and ...format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included...CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening Cas9...engineering approach. Figure 1: Overview of the basic CRISPR mechanism Engineered CRISPR systems contain... genome-wide screens. This guide will provide a basic understanding of CRISPR biology, introduce the various..., efficiency, or flexibility of the Cas enzyme. Basic Mechanisms of CRISPR The requirements for a CRISPR...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...

...Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. If you are looking...Molecular Biology Reference Jump To: Molecular Biology Basics Origins of Molecular Genetics Plasmid Elements ...combination of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule...specifically engineered plasmids, commonly referred to as vectors, which have become possibly the most ubiquitous...facilitate the cloning of DNA fragments. Cloning vectors tend to be very simple, often containing only a...expression (for the purposes of gene study). Expression vectors must contain a promoter sequence, a transcription...the transcription process to stop. An expression vector can also include an enhancer sequence which increases...