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We narrowed to 2 results for: cas9 single guide rna sgrna expression vector

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  1. CRISPR Guide

    Type
    Guide
    ...Science Guides CRISPR Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large...components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short...conversions using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target...more about Cas9 multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease...transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which Cas9 is included in the gRNA-containing...We’ll discuss more Cas9 fusion tools later in the guide. Increasing fidelity of Cas9 Researchers have also... available with multiple Cas9 variants, including high fidelity Cas9s, Cas9s with various PAM requirements...

  2. Lentiviral Vector Guide

    Type
    Guide
    ... Guides Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral...gamma-retroviruses, see Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses typically...Lentiviral Vectors Due to their integration and long-term expression of a transgene, lentiviral vectors are ...Viral Vectors 101 topics More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols...gene silencing by RNAi in primary cells. RNA , 9 (4), 493–501. https://doi.org/10.1261/rna.2192803 PMID: ...lentiviral vectors that can be safely used in laboratory settings. These engineered lentiviral vectors have ...models. Lentiviral vectors can target non-dividing cells, enable stable long-term expression in a host, and...
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