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We narrowed to 6 results for: gfp fluorescence reporter

Showing: 1 - 6 of 6 results

  1. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker...antibiotic, fluorescence-activated cell sorting (FACS) is used to sort cells expressing GFP and later, ...

  2. Modular Cloning Guide

    Type
    Guide
    ...Sieber Plasmids with control elements and fluorescent reporters to be used in combination with the MoClo-YTK...PhytoBrick parts. MoBiFC - Modular Bimolecular Fluorescence Complementation Toolkit Plant Expression Benjamin...Field 50 plasmids for assembling bimolecular fluorescence complementation experiments to analyze protein-protein...synthetic terminators, protein purification tags and fluorescence proteins for use in E. coli for a variety of...a combinatorial library of constructs expressing GFP at 18 levels for rapid characterization in new organisms...Christopher Dupont 95 plasmids including backbones, fluorescent proteins, protein tags, and signal peptides, ...

  3. Molecular Biology Reference

    Type
    Guide
    ...elements. These plasmids contain a reporter gene (e.g., luciferase or GFP) that offers a readout of the activity...the control of a non-bacterial promoter or a fluorescent protein to select or sort the cells by visualization...TALEN Plasmids and Kits , Genome Engineering Kits Reporter Plasmids Used for studying the function of genetic...level of transcription driven by that promoter. Fluorescent Protein Plasmids , Luciferase Plasmids Viral ...relies on the random incorporation of modified, fluorescently-tagged bases during in vitro DNA replication... DNA because the ultimate goal is to have a fluorescently-tagged nucleotide at each position in the DNA...DNA strands are separated by size, read using fluorescence detection, and the sequence outputted in the...

  4. Lentiviral Vector Guide

    Type
    Guide
    ...the only type of selectable marker. Fluorescent reporters, such as GFP, are another common selection marker...antibiotic, fluorescence-activated cell sorting (FACS) is used to sort cells expressing GFP and later expanded...

  5. Optogenetics Guide

    Type
    Guide
    ...of proteins) and sensors (genetically-encoded reporters of molecular signals). These areas have each since...plasmids allow cloning of protein coding sequences with GFP-LOVpep, cpPDZ, ePDZb and ePDZb1 as tags. pDawn/pDusk...activator FixJ in the absence of blue light. 470 Fluorescent Protein domains Dronpa based Light-inducible ...engineered from a green-to-red photoconvertible fluorescent protein 400 UVR8 domains UVR8 plant photoreceptor...

  6. CRISPR Guide

    Type
    Guide
    ...sequences using a fluorescent reporter. In SHERLOCK, a quenched ssRNA fluorescent reporter is added to the...catalytically inactive dCas9 to a fluorescent marker like green fluorescent protein (GFP), creating a customizable...viral genome, and a ssDNA-fluorescently quenched reporter is added. As this reporter is degraded by Cas12a...customizable DNA or RNA label for fluorescence microscopy. RNA aptamers, like MS2 or PP7, can also be fused to...to the gRNA to recruit fluorescently-tagged RNA-binding proteins (RBPs). Multicolor CRISPR imaging can...dCas9 and S. aureus dCas9) tagged with different fluorescent proteins or by fusing the gRNAs to orthogonal...imaging of genomic loci, as in the CRISPR-Sirius . Fluorescent CRISPR systems have been used for dynamic tracking...
Showing: 1 - 6 of 6 results