Adeno-associated virus (AAV) Guide
Type
Guide
...different plasmid. In order to produce rAAV vectors, three plasmids are needed: Transfer plasmid (also known...the packaging plasmid. For a summary of all AAV plasmid components, see the AAV Plasmid Elements table...as cis plasmid) — containing the transgene of interest between the two ITRs. Packaging plasmid (also known...chosen plasmid is compatible with cloning methods available in your lab. When cloning your plasmids, be ...Production The three production plasmids (transfer, packaging, and helper plasmid) are transfected into cells...known to cause disease in humans. Most, if not all of Addgene’s transfer plasmids are replication deficient...Blog Parts of the AAV Transfer Plasmid Parts of the AAV Packaging Plasmid Producing Your rAAV AAV Serotypes...