... AAV replication. The transfer plasmid, Rep/Cap, and the helper plasmid are transfected into HEK293 cells...double-stranded circular episome through double stranded synthesis. These episomes can concatemerize, producing...particles are thought to be maintained in episomes or concatemers. Episomes differ profoundly from viral particles...Resources AAV Plasmids Available at Addgene Biosafety Guide Parts of an AAV Transfer Plasmid Video Web References...Learn about the types of plasmids used to produce AAV in the lab. Addgene’s AAV Guide will teach you ...capsid proteins). When constructing an AAV transfer plasmid, the transgene is placed between the two ITRs, ...addition to Rep and Cap, AAV requires a helper plasmid containing genes from adenovirus. These genes (...