We narrowed to 2 results for: human sgrna library
-
TypeGuide... lentiviral pooled libraries . Browse Libraries: CRISPR Pooled Libraries Cas9 Fusion Tools dCas9 cannot...lentiviral CRISPR libraries. Figure 8: Overview of genome-wide screening using CRISPR libraries What Are Pooled...Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous population of..., so CRISPR libraries contain thousands of unique gRNAs. gRNA design for CRISPR libraries is usually optimized...activation and repression libraries target promoter or enhancer regions. As each library is unique, be sure ...sure to check the library information and original publication to see if a library is suitable for your experiment...CRISPR libraries have been developed for inhibition using dCas9. How Do You Use a CRISPR Library? CRISPR...
-
Lentiviral Vector Guide
TypeGuide...species. Some common types of lentivirus include Human immunodeficiency virus (HIV), bovine immunodeficiency...plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs; 8–10 kb packaging...process requires a production cell line, typically human embryonic kidney 293T (HEK293T) cells. Standard ... an extensive collection of lentiviral CRISPR libraries, including knockout, inhibition/activation, and...editing. Browse Addgene's collection of CRISPR libraries . Cellular Barcoding Much like genomic screens... Inheritance of the barcodes makes lentiviral libraries popular for lineage tracing experiments and monitoring...tumors. Browse Addgene's collection of barcoding libraries . Mouse Modeling Since the development of safer...