Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 5 results for: lentiviral infection

Showing: 1 - 5 of 5 results

  1. Lentiviral Vector Guide

    Type
    Guide
    ... Guides Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...gamma-retroviruses can only infect mitotically active cell types. This means that lentiviruses can infect a greater variety... Things Lentivirus Lentiviral Vector Uses and Overview Overcoming the Challenges of Lentiviral Production...safety. For a summary of all lentiviral plasmid components, see the Lentiviral Plasmid Elements table . In... a wide range of infectivity for different cell types (known as tropism). Lentiviral vectors have gone...different generations. Lentiviral Generations First-generation First-generation lentiviral plasmids are largely...

  2. Gamma-Retroviral Vector Guide

    Type
    Guide
    ...gamma-retroviruses can only infect mitotically active cell types. This means that lentiviruses can infect a greater variety...plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide...difference between lentiviruses and gamma-retroviruses is that lentiviruses are capable of infecting non-dividing... to the Retroviridae family. Gamma-retrovirus infection is associated with many serious illnesses. Common...gamma-retroviruses may not be efficiently packaged by lentiviral packaging systems, and vice versa. Gamma-retroviral...packaging gene env ; usually VSV-G due to wide infectivity VSV-G (vesicular stomatitis virus G protein) ...common envelope gene, due to a wide range of infectivity for different cell types (known as tropism). ...

  3. Guide to Using Pooled Libraries

    Type
    Guide
    ... are infected at a very low multiplicity of infection (MOI) to increase the odds that an infected cell...cells of interest. With others, notably pooled lentiviral plasmid libraries, the plasmids must first be...adequately represented in the population, you’ll need to infect many more cells than the number of plasmids present...Examples of published selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-cancer...delivering as virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells... do not survive the selection mechanism. You’ll infect two sets of cells and subject one set to selection...libraries! Genome-wide Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...

  4. Molecular Biology Reference

    Type
    Guide
    ...particles, such as lentiviral, retroviral, AAV, or adenoviral particles, that can infect your target cells...viral service offers select ready-made AAV and lentiviral particles. Visit our viral service page to learn...

  5. CRISPR Guide

    Type
    Guide
    ...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable...genome-wide screens using CRISPR involves pooled lentiviral CRISPR libraries. Figure 8: Overview of genome-wide...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Figure 8A), then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come ...library is suitable for your experiment. Although lentiviral libraries are the most popular method for CRISPR...our pooled library guide and our blog post on lentiviral pooled libraries . Browse Libraries: CRISPR Pooled...
Showing: 1 - 5 of 5 results