... Guides Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral Vectors Lentiviral Generations...More Lentiviral Vector topics Addgene Protocols pLKO.1 - TRC Cloning Vector Protocols Lentiviral Vector...Addgene's gamma-retroviral vector guide . Lentiviral Vectors The genome of lentiviruses typically ranges from... Things Lentivirus Lentiviral Vector Uses and Overview Overcoming the Challenges of Lentiviral Production...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...Second-generation lentiviral plasmids greatly improved the safety of lentiviral vector production by removing...between second- and third-generation lentiviral plasmids. Lentiviral Vector Production Cloning Cloning your...

...plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide . Gamma-Retroviral...Gamma-Retroviral Vector Guide Gamma-Retroviral Vector Guide Jump To: Gamma-Retroviral Vectors Vector Production...gamma-retroviral vectors is essentially the same as lentiviral vectors. Many gamma-retroviral vectors have selectable...Much like lentiviral vectors, gamma-retroviral vectors integrate at random locations throughout the genome... for lentiviral vectors, but a similar method can be applied when using gamma-retroviral vectors. Gene...of Viruses Viral Vector Elements More Viral Vectors 101 topics More Retroviral Vector topics Addgene protocols...contains many viral vector-specific terms and acronyms, so if you're new to viral vectors or simply need a...

...cells of interest. With others, notably pooled lentiviral plasmid libraries, the plasmids must first be...Examples of published selection mechanisms for lentiviral CRISPR libraries include: resistance to the anti-cancer...delivering as virus, make virus; this creates a pooled lentiviral CRISPR library. Apply pooled library to cells...genome-wide screening with CRISPR/Cas9 . Browse our Viral Vector resources . Check out our blog posts featuring ...libraries! Genome-wide Screening Using CRISPR/Cas9 Lentiviral CRISPR Libraries Enable Genome-Scale, Knockout...

...efficiency Lentiviral transduction Cas9 and gRNA can be present in a single lentiviral transfer vector or separate...components to make lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable,...Components of System Application Mammalian expression vector Cas enzyme promoter can be constitutive (CMV, EF1alpha...lines (for details, see Plasmids 101: Mammalian Vectors ) Transient or stable expression of Cas9 and/or...separate transfer vectors May contain reporter gene (e.g. GFP) or selection marker to identify and enrich...CRISPR elements are inserted into an AAV transfer vector and used to generate AAV particles (for details...oligos and clone these oligos into your desired vector. In many cases, targeting oligos are synthesized...

...pooled format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector systems, in which...CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries are a heterogeneous...heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains an individual...Cas9-based genome engineering from a single lentiviral vector. Nucleic Acids Research , 42 (19), e147. PMID...8E). In a 2-vector system, you’ll need to either co-infect with a Cas9-producing lentivirus or use a Cas9...targeting in the double digits. These multiplex gRNA vectors can be used to knock out, activate, or repress ...multiplexing . Browse Plasmids: Multiplex gRNA Vectors Engineering the Cas9 Endonuclease CRISPR specificity...

...Commonly used cloning vectors include Gateway entry vectors and TOPO cloning vectors. If you are looking...combination of a DNA fragment with a plasmid or vector DNA backbone generates a recombinant DNA molecule...specifically engineered plasmids, commonly referred to as vectors, which have become possibly the most ubiquitous...facilitate the cloning of DNA fragments. Cloning vectors tend to be very simple, often containing only a...expression (for the purposes of gene study). Expression vectors must contain a promoter sequence, a transcription...the transcription process to stop. An expression vector can also include an enhancer sequence which increases... amount of protein or RNA produced. Expression vectors can drive expression in various cell types (mammalian...

...Chemogenetics CRISPR Optogenetics Pooled Libraries Viral Vectors Antibodies Produced by the immune system, antibodies...restricted to certain gene sets. Read More Viral Vectors Find educational guides to learn more about the...that are commonly used in research, including: Lentivirus Adeno-associated Virus (AAV) Adenovirus γ-Retrovirus...