...isoforms of these packaging components. Therefore, lentivirus may not be efficiently packaged by gamma-retroviral...flanked by LTRs; 8–10 kb packaging capacity Packaging plasmid — contains packaging genes gag and pol , and...tat gene Can be packaged by both second- and third-generation packaging systems Packaging Plasmid One plasmid...plasmids available at Addgene. Lentivirus Versus Gamma-Retrovirus Lentiviruses and gamma-retroviruses fall... the use of “retro”. Lentiviruses have more complex genomes, containing packaging genes and accessory ...contain packaging genes. From an experimental standpoint, the main difference between lentiviruses and gamma-retroviruses...that lentiviruses can infect a greater variety of cell types than retroviruses. Both lentiviruses and ...

...flanked by LTRs; ~8 kb packaging capacity Packaging plasmid — contains packaging genes gag and pol Envelope...available at Addgene. Gamma-Retrovirus Versus Lentivirus Lentiviruses and gamma-retroviruses fall under the Retroviridae...genomes, containing only the necessary packaging genes, while lentiviruses also contain accessory genes specific...main difference between lentiviruses and gamma-retroviruses is that lentiviruses are capable of infecting...that lentiviruses can infect a greater variety of cell types than retroviruses. Both lentiviruses and ...of these packaging components. Therefore, gamma-retroviruses may not be efficiently packaged by lentiviral...to lentiviral packaging methods . The three plasmids described above (envelope, packaging, and transfer...

...lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable, tunable expression ... marker to identify and enrich positive cells Packaging and envelope plasmids provide the necessary components...particles (for details, see our AAV Guide ) ∼4.5 kb packaging limit (only compatible with smaller Cas enzymes...

...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...function. Its size allows it to be efficiently packaged into adeno-associated viruses (AAVs) . Other natural...delivery to cells that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available...as liquid DNA, or in select cases, as pre-made lentivirus . DNA libraries are shipped at a concentration...need to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After...editing capabilities but are small enough to be packaged in AAV particles. Cas13 fusions have also been...