...plasmids available at Addgene. Lentivirus Versus Gamma-Retrovirus Lentiviruses and gamma-retroviruses fall...main difference between lentiviruses and gamma-retroviruses is that lentiviruses are capable of infecting...that lentiviruses can infect a greater variety of cell types than retroviruses. Both lentiviruses and ...second-generation transfer plasmids as well. Third-generation plasmids include (Figure 4): Transfer plasmid — ...activate the promoter on the transfer plasmid. Many of the lentiviral transfer plasmids that have been deposited...Uses Safety Resources Plasmid Elements Glossary Lentiviruses are a type of retrovirus and belong to the Retroviridae... Retroviridae family. Infection with lentiviruses has a long incubation period that eventually leads to...

...available at Addgene. Gamma-Retrovirus Versus Lentivirus Lentiviruses and gamma-retroviruses fall under the Retroviridae...main difference between lentiviruses and gamma-retroviruses is that lentiviruses are capable of infecting...that lentiviruses can infect a greater variety of cell types than retroviruses. Both lentiviruses and ...plasmids containing the transfer, envelope, or packaging components. The transfer plasmid encodes the gene...containing only the necessary packaging genes, while lentiviruses also contain accessory genes specific to each...plasmids, as lentiviral vectors do. For more about lentiviruses, see Addgene's lentiviral vector guide . Gamma-Retroviral...gamma-retroviral vectors, you need three plasmids (Figure 2): Transfer plasmid — contains transgene, sgRNA, or shRNA ...

...be present in a single lentiviral transfer vector or separate transfer vectors May contain reporter gene...lentiviral particles (for details about lentivirus, see our Lentivirus Guide ) Stable, tunable expression ...transduction CRISPR elements are inserted into an AAV transfer vector and used to generate AAV particles (for...

...need to generate lentivirus containing the entire library (Figure 8D). This lentivirus will then be used...libraries are a heterogeneous population of lentiviral transfer vectors. In most cases, each plasmid contains ...then cloned in a pooled format into lentiviral transfer vectors (Figure 8B). Libraries come in 1-vector...delivery to cells that are poorly transduced by lentivirus. Non-mammalian CRISPR libraries are also available...as liquid DNA, or in select cases, as pre-made lentivirus . DNA libraries are shipped at a concentration...need to either co-infect with a Cas9-producing lentivirus or use a Cas9-expressing stable cell line. After...short 3′ overhang, allowing for directional gene transfer, which may increase the efficiency of gene editing...