Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 6 results for: plasmid for e coli

Showing: 1 - 6 of 6 results

  1. Molecular Biology Reference

    Type
    Guide
    ...Molecular Genetics Plasmid Elements Working with Plasmids Introduction Types of Plasmids E. coli strains for ...prior to use in an experiment. E. coli strains for propagating plasmids E. coli are gram-negative, rod shaped...Restriction Cloning Plasmids 101: Inducible Promoters Plasmids 101: What is a plasmid? Plasmids 101: Antibiotic...Addgene blog posts relating to common E. coli lab strains and E. coli strains specialized for protein expression...propagating plasmids Antibiotics commonly used for plasmid selection DNA sequencing for plasmid verification...to be studied into the plasmid. Below are some common plasmid elements: Plasmid Element Description Origin...biology basics and plasmid components. Plasmids 101: Origin of Replication Plasmids 101: The Promoter ...

  2. Sequencing Primers

    Type
    Guide
    ... For sequencing plasmids in our repository, we've chosen primers based on the plasmid backbone and insert...ATGCCATAGCATTTTTATCC (Invitrogen) For vectors with E. coli araBAD promoter, forward primer pBAD Reverse GATTTAATCTGTATCAGG...GATTTAATCTGTATCAGG (Invitrogen) For vectors with E. coli araBAD promoter, reverse primer pBluescriptKS TCGAGGTCGACGGTATC...uses for sequence verification of deposited plasmids. Plasmid... Plasmid Reference Molecular Biology Reference Sequencing... useful in your sequencing reaction, find your plasmid page and see what primers are listed under "5' ... for sanger sequence verification of deposited plasmids. Below is a list of commonly used primers. This...

  3. Adenovirus Guide

    Type
    Guide
    ...you generally need two plasmids: Shuttle plasmid (also known as transfer plasmid) — containing the transgene...shuttle plasmid. For a summary of all adenoviral plasmid components, see the Adenoviral Plasmid Elements...are BJ5183 E. coli cells containing pAdEasy. pAdEasy is a ~33 kb adenoviral backbone plasmid containing...AdEasier-1 cells BJ5183 E. coli cells containing the pAdEasy-1 packaging plasmid. Adenovirus (AdV) Wildtype...for recombination with the adenoviral plasmid. Adenoviral plasmid — containing the rest of the adenoviral...chosen plasmid is compatible with cloning methods available in your lab. When cloning your plasmids, be ...consists of two plasmids that eventually recombine to form one: Shuttle/transfer plasmid (e.g. pAdTrack...

  4. CRISPR Guide

    Type
    Guide
    ...36593413 Vo, P. L. H., Ronda, C., Klompe, S. E., Chen, E. E., Acree, C., Wang, H. H., & Sternberg, S. H... R., Hanna, R. E., Hegde, M., Donovan, K. F., Strand, C., Sullender, M. E., Vaimberg, E. W., Goodale, ... the DNA and replaces it with another base. In E. coli , this strategy yields C to A base edits, whereas...harnessed for antiviral mechanisms in bacteria ( E. coli ). Like the other Cas enzymes, the Cascade-Cas3...edits. PASSIGE ( P rime- E diting- A ssisted S ite- S pecific I ntegrase G ene E diting), developed by the.... PMID: 26735016 Lee, J. K., Jeong, E., Lee, J., Jung, M., Shin, E., Kim, Y., Lee, K., Jung, I., Kim, ..., K. M., Aach, J., Guell, M., DiCarlo, J. E., Norville, J. E., & Church, G. M. (2013). RNA-Guided Human...

  5. Promoters

    Type
    Guide
    ...biology basics and plasmid components. Plasmids 101: The Promoter Region-Let's Go Plasmids 101: Inducible ... popular Plasmids 101 series , covers topics ranging from the newest breakthroughs in plasmid technologies...Inducible Promoters Plasmids 101: Repressible Promoters Plasmids 101: Terminators and PolyA signals...operon trp Repressible by tryptophan Promoter from E. coli tryptophan operon Ptac Regulated like the lac promoter... Plasmid Reference Molecular Biology Reference Promoters...

  6. Cloning

    Type
    Guide
    ... of interest (YGOI) into a recipient plasmid. (Image from Plasmid 101: Restriction cloning ) Back to Top...product into a plasmid. The major disadvantage of TOPO® cloning is that very few plasmid backbones are ...Entry plasmids are also available, allowing for rapid cloning of PCR products into donor plasmids without.... The product may be transformed directly into E. coli , where the nicks will be repaired by the normal...manipulate genes of interest and then insert them into plasmids for replication and protein expression. So how...fragments. Given its prevalence, the vast majority of plasmids used for DNA cloning and expression contain several...restriction sites on either side of it into any plasmid that has the same sites in the same orientation...
Showing: 1 - 6 of 6 results