Lentiviral Vector Guide
Type
Guide
...second-generation transfer plasmids as well. Third-generation plasmids include (Figure 4): Transfer plasmid — contains...Packaging plasmid 1 — contains gag and pol Packaging plasmid 2 — contains rev Envelope plasmid — contains...systems Packaging Plasmid One plasmid encoding gag , pol , tat , and rev Two plasmids, one encoding gag...distinct plasmids for safety. For a summary of all lentiviral plasmid components, see the Lentiviral Plasmid...produce lentiviral vectors, you need three (or four, for third generation) plasmids: Transfer plasmid — contains...removed Envelope plasmid — contains env Figure 2: First-generation lentiviral plasmids. Created with BioRender.com... promoter on the transfer plasmid. Many of the lentiviral transfer plasmids that have been deposited with...