...destination vector (in this case the destination vector would be the lentiviral transfer vector). The destination...cloning vector pLKO.1. Trono Lab Resources: Lentivectors Toolbox and Maps Standard Lentiviral Production...plasmid must be modified. Most shRNA‐expressing lentiviral plasmids, such as pLKO.1, use a U6 or H1 promoter...later be digested from this new vector and cloned into the lentiviral vector. This is basically shuffling...site (MCS) from a separate vector into the single site in the lentiviral vector and generate more useful... transfer vector during the viral production stage. Many of the lentiviral transfer vectors that have ... most lentiviral vectors. Packaging Gag in trans Precursor structural protein of the lentiviral particle...