... within bacteria, while allowing for selection against any bacteria not carrying the plasmid. Additionally...out-populate bacteria with plasmids, thus selecting against the propagation of these plasmids through cell ...frequently accomplished through expression of an shRNA targeting the mRNA of the gene of interest. These...plasmids contain a reporter gene (for example, luciferase or GFP) that offers a read-out of the activity...of interest could be inserted upstream of the luciferase gene to determine the level of transcription ...