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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3 results for: single guide rna sgrna

Showing: 1 - 3 of 3 results

  1. CRISPR Guide

    Type
    Guide
    ...Science Guides CRISPR Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large...components: a guide RNA ( gRNA or sgRNA ) and a CRISPR-associated endonuclease (Cas enzyme). The gRNA is a short...conversions using a prime editing guide RNA ( pegRNA ). Like sgRNA, pegRNA directs the nickase to the target...that target RNA instead of DNA enables tracking of RNA in live cells, including orthogonal RNA/DNA labeling...Koonin, E. V., & Zhang, F. (2015). CPF1 is a single RNA-Guided endonuclease of a Class 2 CRISPR-CAS system...libraries use arrays of multiple gRNAs to target paralogs or lncRNAs . Guide RNAs are designed in silico and...modified scaffold gRNA and additional RNA-binding helper activators; highest levels of single-gene activation...

  2. Gamma-Retroviral Vector Guide

    Type
    Guide
    ... Science Guides Viral Vectors Gamma-Retroviral Vector Guide Gamma-Retroviral Vector Guide Jump To: Gamma-Retroviral... The genome of retroviruses is made of RNA. This retroviral RNA is reverse transcribed into DNA before...gamma-retroviruses ranges from 9–11 kb, encoded on RNA (Figure 1). This RNA is reverse transcribed into the provirus...will be integrated into a host genome. This single strand of RNA contains packaging genes and the long-terminal...2): Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs; ~8 kb packaging...Read our gamma-retroviral guide to learn about gamma-retroviral components, retroviral production, and...expression in a host and low immunogenicity. This guide contains many viral vector-specific terms and acronyms...

  3. Lentiviral Vector Guide

    Type
    Guide
    ... Science Guides Viral Vectors Lentiviral Vector Guide Lentiviral Vector Guide Jump To: Lentiviral...gene silencing by RNAi in primary cells. RNA , 9 (4), 493–501. https://doi.org/10.1261/rna.2192803 PMID: ...genome of viruses in this family is made of RNA. This retroviral RNA is reverse transcribed into DNA before...typically ranges from 8–10 kb, encoded on RNA (Figure 1). This RNA is reverse transcribed into the provirus...will be integrated into a host genome. This single strand of RNA contains packaging genes, regulatory genes...plasmids: Transfer plasmid — contains transgene, sgRNA, or shRNA of interest flanked by LTRs; 8–10 kb packaging...Read our lentiviral guide to learn about lentiviral components, generations, lentiviral production, and...
Showing: 1 - 3 of 3 results