Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 35 results for: MAK

Showing: 11 - 20 of 35 results

  1. Gibson Assembly Protocol

    Type
    Protocol
    ... with a two-part Gibson reaction if you're only making a small change in a plasmid (such as point mutations...manufacturer's instructions. You can purchase master mix or make your own. Transform bacteria with the DNA and screen...overlapping PCR primers (>60 bp) but too short to make its own part (<150 bp). Pro-Tip Please note that...

  2. Using a Light Microscope Protocol

    Type
    Protocol
    ...of a microscope are magnification (the ability to make an image larger) and resolution (the ability to ...through the sample. However, some of that light won’t make it through the sample because different components...fine focus knob (the smaller of the focus knobs) to make minor adjustments to the focus. After you are happy...

  3. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ... of the rotor and place them in a stable rack. **Make sure not to disturb the gradient!** Collect Fractions...flow from the needle set at the 40–60% interface. Make sure that the microcentrifuge tubes are well positioned...spin, add more formulation buffer and sample and make sure to pipet back and forth a few times to mix ...

  4. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ... mouse genes. Addgene is working with the TRC to make this shRNA cloning vector available to the scientific... microfuge tubes (do NOT use polystyrene tubes), make a cocktail for each transfection: 1 μg pLKO.1 shRNA...scientists with high-quality goods and services. Addgene makes every effort to ensure the accuracy of its literature...typographical or other errors may occur. Addgene makes no warranty of any kind regarding the contents of...

  5. AAV ddPCR Titration

    Type
    Protocol
    ...the risk of contaminating reagents we recommend making small aliquots of master mixes, primers, and probes...and place in the Biological Safety Cabinet (BSC). Make sure that the BSC to be used for dilution and the...wells. Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge ...

  6. Lentivirus ddPCR Titration

    Type
    Protocol
    ...place it in the Biological Safety Cabinet (BSC). Make sure that the BSC is supplied with sufficient pipette...wells. Cover the cartridge with the DG8 gasket, making sure that it is secure. Transfer the cartridge ...the risk of contaminating reagents we recommend making small aliquots of master mixes, and primers/probe...

  7. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...Over-Agar Antibiotic Plating You may also like... Making LB Agar Plates Bacterial Transformation Recovering...transformed E. coli . Over-agar spreading of antibiotic makes it easy for an investigator to conveniently plate...

  8. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ... Rule For each sample you want to load on a gel, make 10% more volume than needed because several microliters...For example, if you want to load 1.0 μg in 10μL, make 1.1μg in 11μL. Reference Page Top Index...

  9. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...sequencing primers, including: Is the primer unique? Make sure the primer only anneals once within the entire...incorrect sequence. Look at the peaks in the area and make sure they are justifiable peaks. For instance, ...

  10. Protocol - Bacterial Transformation

    Type
    Protocol
    ... that increase the transformation efficiency and make bacteria more susceptible to either chemical or ... plasmids at once. To save money, many labs also make their own competent cells. This is a relatively ...
Showing: 11 - 20 of 35 results