Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 44 results for: APP

Showing: 21 - 40 of 44 results

  1. Lentivirus ddPCR Titration

    Type
    Protocol
    ...complete: Prepare 7 microcentrifuge tubes with the appropriate amount of media volume listed in the chart below...tube. Add 4 µL of the 25 ng/µL samples to the appropriate PCR tubes. Pipette back and forth 10 times to...the average infectious titer obtained from the appropriate dilutions to calculate the final infectious titer...

  2. Protocol - How to Perform Sequence Analysis

    Type
    Protocol
    ...within the entire construct. Is the primer an appropriate distance from the target? The primer should be... should I do? Check your trace file first; the apparent mismatch/mutation may be the result of a mis-called...

  3. Water Bath Protocol

    Type
    Protocol
    ...in place. Turn the water bath on and set the appropriate temperature as described in your protocol. How...in step 3. You will also need to maintain the appropriate water level in the water bath so before use, ...

  4. Ligation Independent Cloning

    Type
    Protocol
    ...traditional cloning, base-pairing in the short overlapping regions (usually 4 bp) does not provide enough...with the start codon or tag sequences (where appropriate). The primer length is dependent on the T4 Pol...

  5. Gibson Assembly Protocol

    Type
    Protocol
    ...fragment length or end compatibility, multiple overlapping DNA fragments can be joined in a single isothermal...part to be inserted is too long to include on overlapping PCR primers (>60 bp) but too short to make its...

  6. Lentivirus Production

    Type
    Protocol
    ...lentivirus can be used for a variety of downstream applications such as stable-cell line generation. Workflow...mL of DMEM Complete to a 15 mL conical and an appropriate amount of 25 mM chloroquine to the conical so...

  7. Colony Formation Titering Assay

    Type
    Protocol
    .... Add 1.5 mL of DMEM complete containing the appropriate antibiotic. Incubate the cells for ~2 weeks. ...replace it with fresh DMEM complete containing the appropriate antibiotic. When the untransduced (negative control...

  8. Pipetting Protocol

    Type
    Protocol
    ... P1000. The table shows the pipette with the appropriate dispense volume: This image shows the P2, P10...,000 µL Each pipette should be used with the appropriate tips, which are made in a number of sizes and...

  10. Western Blot

    Type
    Protocol
    ... can be adapted for wet and semi-dry transfer approaches. Last Update: January 24, 2022 Workflow Timeline...remove supernatant. Resuspend cell pellet in appropriate volume of cold lysis buffer. Pro-Tips The volume...

  11. AAV ddPCR Titration

    Type
    Protocol
    ...Water, Hyclone, SH30538.02 GeneAmp 10X PCR Buffer, Applied Biosystems, N8080129 ddPCR Supermix for Probes ... PCR tubes. Add 5 µL of dilutions 6–8 to the appropriate PCR tubes. Pipette back and forth 5 times. Lightly...

  12. Kit Free RNA Extraction

    Type
    Protocol
    ... not affect the quality of RNA or downstream applications. To improve yield of RNA, instead of incubating...

  17. Fluorescence Titering Assay

    Type
    Protocol
    ... these cells can still be used in downstream applications. Safety Warnings Lentivirus is generally considered...

  18. General Transfection

    Type
    Protocol
    ...), but any HEK293T cell line should work. This approach can be adapted for different cell lines and different...
Showing: 21 - 40 of 44 results