We narrowed to 29 results for: BASE

1. Coomassie Purity Stain of Recombinant Antibodies

   Type

   Protocol

   ... to determine the concentration of your samples based on your sample’s HC + LC area. Example calculation...

2. Pouring LB Agar Plates

   Type

   Protocol

   ... make. The precise mass you measure out will be based on the number of plates you’d like to pour. For ...

3. AAV Titration by qPCR Using SYBR Green Technology

   Type

   Protocol

   ...physical titer of samples (viral genomes (vg)/mL) based on the standard curve and the sample dilutions. ...efficiency of PCR) ~100% (90%–110% range is acceptable) Baseline removal: all samples will have some small amount...

4. Isolating a Monoclonal Cell Population by Limiting Dilution

   Type

   Protocol

   ...monoclonal lines generated using this protocol will vary based on several factors, including the growth properties...

5. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

   Type

   Protocol

   ... 2: Buffer exchange Choose Option 1 or Option 2 based on the concentration of the pooled sample above....

6. AAV ddPCR Titration

   Type

   Protocol

   ...The dilution series outlined in this protocol are based on an AAV titer range of 5E+12–5E+13 GC/mL, where...

7. CRISPR Library Amplification

   Type

   Protocol

   ... but care must be taken to perform adequate NGS based QC to ensure no change in representation compared...

8. Lentivirus ddPCR Titration

   Type

   Protocol

   ...The dilution series outlined in this protocol is based on an LV titer range of 1E+05–1E+09 TU/mL, where...

9. Gibson Assembly Protocol

   Type

   Protocol

   ...assembly of DNA molecules up to several hundred kilobases. Nature Methods , 6(5), 343–345. https://doi.org...