...Scientific, 10199-452 Ice bucket 96-well freezer blocks (x 3) Reagents Molecular Biology Grade Water, Hyclone, ...20X): 5 µL in 95 µL 1X PCR buffer (1:400) Dilution 3 (20X): 5 µL in 95 µL 1X PCR buffer (1:8,000) Dilution...dilution BSC. Prepare 1X dilution buffer (see recipe above). Save ~25 µL of 1X dilution buffer in a microcentrifuge...

...stable cell pool were then expanded for an additional 3 weeks under blasticidin selection. Anti-Cas9 Western...approximately 50–60% confluent. For 293T cells this is about 2 × 10 6 cells in a 10 cm dish. Each 10 cm dish ...cell solution into the conditioned medium prepared above to make a new cell solution at a concentration of...

...hand for 10 seconds. Incubate the sample(s) for 2-3 minutes on ice and centrifuge for 15 minutes at 12,000... working with the volatile reagents in the list above. Procedure Option #1 - Solution D Protocol Before...sure to prepare solution D (see reagent section above for the recipe). If using TRIzol®, jump down to ...