We narrowed to 25 results for: tec

1. Handling Plasmids from Addgene - Purifying Plasmid DNA

   Type

   Protocol

   ...Bacterial Culture Introduction Many molecular biology techniques require highly purified and concentrated plasmid...

2. What is Polymerase Chain Reaction (PCR)

   Type

   Protocol

   ...between the oligo primers. This molecular biology technique creates several micrograms of target DNA from ...

3. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

   Type

   Protocol

   ... is toxic. When handling, use proper personal protective equipment including laboratory coat, laboratory...

4. AAV Production in HEK293 Cells

   Type

   Protocol

   ...meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents Adherent HEK293T cells (ideally...

5. CRISPR Library Amplification

   Type

   Protocol

   ...to lysis by freeze-thaw if not suspended in cryoprotectant like Glycerol or DMSO solutions. Quantify the...