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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 49 results for: Ide

Showing: 41 - 49 of 49 results

  1. Colony Formation Titering Assay

    Type
    Protocol
    ...for Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service... in the lab by heating to 56 °C for 30 min. Considerations Before You Start The health of the target cell...dilutions. Pro-Tip The higher dilution wells tend to provide more accurate titers as the likelihood of multiple...

  2. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...Green Technology You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Introduction... 96-well optical plate Pipette tips General Considerations Always run standards and samples in duplicate...small amount of background signal that is most evident during initial PCR cycles. This background signal...

  3. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...Limiting Dilution You may also like... Viral Vector Guides Virus Blog Posts Addgene Protocols Viral Service...monoclonal line could be generated at any time. Considerations Before You Start Some cell lines tend not to... and is described in Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem...

  5. Weighing Reagents Protocol

    Type
    Protocol
    ...Intro to the Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction...of material, use an analytical scale. This will provide more accuracy as these types of scales display ...

  7. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...Pro-Tip DNA resuspension can take time, it is a good idea to let it sit for several hours to overnight at ...at 4°C. Tips and FAQ Plasmid purification kits provide the fastest way to obtain a high concentration ...

  8. DNA Quantification

    Type
    Protocol
    ...affected the accuracy of the NanoDrop. It is a good idea to re-zero any spectrophotometer between samples...

  9. Centrifugation

    Type
    Protocol
    ...Intro to the Lab Bench Check out more protocols and videos to help you get started in the lab! Introduction...
Showing: 41 - 49 of 49 results