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...Protocol for Ligation Independent Cloning... Protocols Ligation Independent Cloning Ligation Independent...Methods Empty Backbone Guide Molecular Biology Protocols Overview Ligation Independent Cloning (LIC) obviates...Addgene's collection of empty LIC cloning vectors Protocol Step 1: Design Your Primers Primer design for ...counter-selection marker (see our restriction digest protocol ). After the digest is complete, you will need...all other nucleotides from standard polymerase protocol), causing the enzyme to perform exonuclease activity... for each transformation, following a standard protocol . Search Addgene's collection of empty LIC cloning...

... Protocols Centrifugation Centrifugation Protocol Intro to the Lab Bench Check...Check out more protocols and videos to help you get started in the lab! Introduction A centrifuge is a ...or keep samples at specific temperatures. This protocol will cover the general procedure and features ...Microfuge tubes or other suitable container Personal protective equipment (PPE) appropriate for the biosafety...and temperature, if applicable) listed in your protocol. Pro-Tip Spin speed is often given as Relative...Centrifuges are important tools for many different protocols. Improper use of a centrifuge can damage the equipment...

...Follow this protocol to produce adeno-associated virus (AAV) in HEK293 cells.... Protocols AAV Production... Molecular Biology Reference Introduction This protocol can be used to produce AAV from one Five Chambers...meter Stir plate Magnetic stir bar Sonicator Ear protection Vortex Reagents Adherent HEK293T cells (ideally...and lots of FBS to find one suitable for your protocols. FBS can be purchased already heat-inactivated...Other transfection reagents may be used in this protocol, but their conditions must be optimized. Plasmids...at 4 °C before proceeding with the purification protocol. Sample Data Figure 1: HEK293T cells at various...

... Virus Blog Posts Mol Bio Protocols Viral Service Introduction This protocol can be used to titer lentivirus...This protocol can be used to titer lentiviral preparations that confer antibiotic resistance. Protocols...of infection (MOI) in downstream studies. This protocol was developed for A549 cells but can be adapted...and lots of FBS to find one suitable with your protocols. FBS can be purchased already heat-inactivated...subjected to multiple freeze-thaw cycles. This protocol outlines the seeding of the cells at the same ...

... on size by gel electrophoresis . Protocol Video Watch the protocol video below to learn how to perform... of Polymerase Chain Reaction with protocol, tips and FAQ Protocols... Protocols Polymerase Chain Reaction (PCR) Polymerase...minutes at 72°C: A final extension to fill-in any protruding ends of the newly synthesized strands. Materials... Primer Design and PCR Design Primers. See our protocol on how to design primers . Note: (Link opens in...primers for detailed instructions and check out our protocol on plasmid cloning by PCR to learn how to design...

... Virus Blog Posts Mol Bio Protocols Introduction The AAV Titration protocol can be used to determine the... Technology, and Addgene’s AAV Titration protocol. Protocols... Protocols AAV Titration by qPCR Using SYBR Green Technology... an AAV prep using SYBR green technology. This protocol was validated using an internal reference AAV ... to those reported by these institutions. This protocol is for a 96-well plate with 20 μL reaction volume...sample to the bottom of the tube. Run the following protocol in your qPCR instrument using SYBR detection: ...

...Follow this protocol to perform digital droplet PCR (ddPCR) for purified AAV titration.... Protocols ddPCR Titration... Molecular Biology Reference Introduction This protocol describes how to use droplet digital PCR (ddPCR...recombinant Adeno-associated viral vectors (AAV). This protocol specifically uses primers and probes targeting... targets. The dilution series outlined in this protocol are based on an AAV titer range of 5E+12–5E+13...blog post on ddPCR for AAV quantification. This protocol was modified from the publication Lock et al. ...pre-chilled 96-well freezer blocks during use. In this protocol, a dilution series is prepared for each AAV sample...

... this protocol to perform digital droplet PCR (ddPCR) for lentivirus vector titration.... Protocols ddPCR...Lentivirus Guide Virus Blog Posts Introduction This protocol describes how to use droplet digital PCR (ddPCR...ddPCR) to titer lentivirus vectors. This protocol specifically uses primers and probes targeting integrated...normalization. The dilution series outlined in this protocol is based on an LV titer range of 1E+05–1E+09 TU...dilutions depending on their particular sample. This protocol was modified from the publication Wang et al. ...control should be close to zero (A01). In this protocol, the lentiviral particles are serially diluted...

...like... Viral Vector Guides Mol Bio Protocols Introduction This protocol can be used to produce lentivirus...Use this protocol to generate lentivirus Protocols... Protocols Lentivirus Production Lentivirus Production You may...cells using a polyethylenimine (PEI) transfection protocol. This procedure can be modified for alternative...and lots of FBS to find one suitable with your protocols. FBS can be purchased already heat inactivated...

...is at 260nm. Protein maximally absorbs at 280nm and the ratio of nucleic acid to protein (260/280) is ...purity using Addgene’s DNA Quantification Protocol. Protocols... Protocols DNA Quantification DNA Quantification You...