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...synaptic cleft proteins are currently under development. Figure 2: Key adhesion proteins and receptor–ligand...particular proteins. Browse or search the table below to find antibodies targeting V5, Protein C, DYKDDDDK...secreted proteins from a collaboration between Addgene and IPI. Antibodies... Antibodies Institute for Protein Innovation...Innovation Antibody Collection Institute for Protein Innovation Antibody Collection Axon Guidance Antibodies... in a new window) Welcome to the Institute for Protein Innovation (IPI) Collection! Here, you’ll find ...IPI to generate antibodies to highly conserved proteins and other difficult-to-target antigens that are...

... Plasmid Collections Fluorescent Protein Guide Fluorescent Proteins: Plasmids and Resources Addgene's...fluorescent proteins and to find plasmids for your next experiment. Browse Fluorescent Protein Plasmids ... Backbones Study your protein of interest by fusing it to a fluorescent protein. Michael Davidson Fluorescent...protein-protein interactions or conformational changes within a protein. Biosensors Monitor small biomolecules...and Explore Addgene Blog: Fluorescent Proteins Fluorescent Protein Plasmid Kits Allen Institute for Cell...to learn about the applications of fluorescent proteins and to find the plasmids that are available from...Addgene's repository contains thousands of fluorescent protein plasmids. Use this guide to learn more about the...

...is often used to study (1) protein-protein interactions where each protein is separately fused to a donor...to report on protein binding or conformational changes. Organic dyes, fluorescent proteins, and other molecules...changes within a protein where the donor and acceptor are both fused to the same protein (also referred ...calibration standards and fusion proteins Feldmann et al. (2023) Protocol for deriving proximity, affinity...and fast-maturing red fluorescent protein Variety of fusion proteins, positive controls for many FRET ...measure protein interactions or conformation changes. Plasmid... Plasmid Collections Fluorescent Proteins FRET Fluorescent...

...Rinehart Lab Phosphoprotein Reagents Rinehart Lab Phosphoprotein Reagents Phosphoprotein Synthesis Reagents...generate the new phosphorylated protein. Protein Phosphorylation Protein phosphorylation is one of the ...positions for testing phosphoproteomics methods, proteomics search algorithms, proteomics phosphosite localization...phosphorylated proteins with one or more phosphorylation sites (Pirman et al., 2015). Improved phosphoprotein production...-P for screening phosphorylation-dependent protein-protein interactions Figure 2: Mode #1 and Mode #2 ...multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics . Nature Methods ...Robust production of recombinant phosphoproteins using cell-free protein synthesis . Nature Communications...

... Collections Fluorescent Proteins Fluorescent Biosensors Fluorescent Proteins: Biosensors Metal Ions Small...GFP-Aequorin Protein) for imaging of Ca 2+ dynamics in endoplasmic reticulum GFP-Aequorin Protein Sensor for... Red fluorescent protein-based cAMP indicator (Pink Flamindo) Red fluorescent protein-based cAMP indicator...pH-sensitive fluorescent protein pHmScarlet . pHmScarlet is a pH-sensitive red fluorescent protein to monitor exocytosis...of Redox-sensitive protein sensors (roGFP) Lifetime imaging of a fluorescent protein sensor reveals surprising...includes BRET-based biosensors of G proteins activated by G-protein-coupled receptors (GPCRs) Yeast GPCR-sensor...Additional Resources Fluorescent biosensors are proteins designed so that their fluorescent properties ...

...Fluorescent Proteins Michael Davidson Fluorescent Protein Collection Michael Davidson Fluorescent Protein Collection...Find fluorescent protein fusion constructs and cloning backbones from the Michael Davidson Lab. Plasmid...collection of ORFs tagged with a variety of fluorescent proteins. The majority of the ORFs are of mammalian origin...different fluorescent tags for you to create fusion proteins with your gene of interest. Most of these plasmids...opens in a new window) for images, tutorials, protocols, and more. Empty Backbones Plasmid Description...photoconvertible, and photoactivatable fluorescent proteins. Plasmid Description Industry Return to top Content...

... Plasmid Collections Fluorescent Proteins In Vivo Imaging Fluorescent Proteins: In Vivo...Additional Resources Fluorescent Proteins: Empty Backbones Fluorescent Proteins: Subcellular Localization Luciferase...fluorescent proteins. Plasmid...Fluorescence microscopy is a powerful tool to study proteins and cells, but imaging in deep-tissue organs or...unique challenges. Genetically-encoded fluorescent proteins most suitable for stable imaging in live animals...background signals. Many near-infrared fluorescent proteins are derived from bacterial phytochrome photoreceptors...vivo imaging tools in this blog post . Far-Red Protein Excitation (nm) Emission (nm) Brightness Plasmids...

...marker protein is fused to EGFP, you may want to fuse your protein to a red fluorescent protein like mCherry...Collections Fluorescent Proteins Subcellular Localization Fluorescent Proteins: Subcellular Localization...pattern, you should fuse your protein of interest with a fluorescent protein that has a different emission...the overlap between your fusion protein and the marker fusion protein using fluorescence microscopy, you...responsible for delivering proteins to the extracellular environment. Proteins are first translated at the...different fluorescent protein markers? Check out the Michael Davidson Fluorescent Protein Collection or search...Michael Davidson Fluorescent Protein Collection Addgene Blog: Fluorescent Proteins Content last reviewed: 10...

...popular fluorescent proteins and empty backbones for expression and cloning of fusion proteins, along with key... Yeast Fluorescent Protein Collection Plasmid Kits Hamdoun Lab Fluorescent Protein Fusion Construct Kit...Which Fluorescent Protein Should I Use? Addgene Blog: Choosing Your Fluorescent Proteins for Multi-Color...create fusion proteins with your gene of interest. Plasmid... Plasmid Collections Fluorescent Proteins Empty Backbones...Backbones Fluorescent Proteins: Empty Backbones Blue/UV Cyan Green Yellow Orange Red Far-Red Near-Infrared...Tables are organized by color of the fluorescent protein. The content for this page was originally compiled...

...done using Protein A, Protein G, or the recombinant Protein A/G, which are bacterial proteins that bind...amounts of protein. Includes: Western blot ELISA Capture - used to isolate specific proteins or protein complexes...quantify, identify, separate, and locate proteins of interest. The protein that an antibody recognizes is called...binding to a specific configuration of a protein or a protein:protein complex. Due to their increased manufacturing...genetically engineered small proteins derived from ankyrin repeat proteins. These are antibody mimics instead...to the protein of interest. Signal strength is determined by how many antibodies bind the protein of interest...amounts of protein. Western blot Western blots use antibodies to identify denatured proteins separated ...

... domain protein 1 2B28, UBXD10 ULBP1 UL16 binding protein 1 RAET1I ULBP2 UL16 binding protein 2 N2DL2,...morphogenetic protein 5 MGC34244 BMP6 bone morphogenetic protein 6 VGR, VGR1 BMP7 bone morphogenetic protein 7 OP...TYROBP TYRO protein tyrosine kinase binding protein DAP12, KARAP, PLOSL ULBP1 UL16 binding protein 1 RAET1I...adaptor-related protein complex 3, beta 1 subunit ADTB3, ADTB3A, HPS, HPS2, PE AZGP1 alpha-2-glycoprotein 1, zinc-binding...shock 70kDa protein 1B FLJ54328, HSP70-1B, HSP70-2, HSPA1A HSPA1L heat shock 70kDa protein 1-like HSP70...HSPA2 heat shock 70kDa protein 2 HSP70-2, HSP70-3 HSPA4 heat shock 70kDa protein 4 APG-2, HS24/P52, MGC131852...

...Visualizing Protein–Protein Interactions Easy Split Fluorescent Proteins for Studying Protein–Protein Interactions...Strains for Protein Expression Protein Expression Epitope Tags Protein Tags Return to top Protein Visualization...that can be used for protein purification, protein visualization, study of protein interactions, controlled...Bacterial Expression Systems Protein Purification Protein Visualization Protein Interactions Controlled Expression...using protease cleavage sites. Other protein tags and signal peptides are used to enhance protein solubility... out more! Protein Purification To study the function, structure, and activity of a protein it is generally...biofactories to express plasmids and produce proteins in high yields. Proteins can then be extracted from the culture...

...the S protein by host cell proteases. The S protein of SARS-CoV-2 is primed by the serine protease TMPRSS2...encoding SARS-CoV-2 proteins, (spike protein, S; small envelope protein, E; nucleocapsid protein, N; and membrane...a cysteine endosomal protease that triggers proteolysis of SARS-CoV Spike protein, activating its membrane...SARS-CoV-2 nucleocapsid protein. Brian Geiss 211757 Anti-SARS-CoV-2 Nucleocapsid Protein [mBG86] Clone mBG86...membrane protein, M), including plasmids for protein production, secretion, and codon-optimized...TMPRSS2 plasmids - a serine protease that primes the SARS-CoV-2 S protein and is involved in virus entry...cleaves precursor proteins to a biologically active state. The SARS-CoV-2 S protein contains a potential...

...binding protein Rac) RAF AFAF BRAF RAF1 Serine/threonine kinases: A-Raf proto-oncogene B-Raf proto-oncogene...GTPase and is a member of the G protein (guanine nucleotide binding protein) family. GTPases serve as molecular...known as MAPK; Mitogen-activated protein kinase ETS ETS1 ETS2 ETS proto-oncogene, transcription factor ...substrate JUN Jun proto-oncogene KSR KSR1 KSR2 Kinase suppressor of ras MDM2 MDM2 proto-oncogene MEK MAP2K1...known as MAP2K; Mitogen-activated protein kinase kinase MET MET proto-oncogene, receptor tyrosine kinase...phosphoinositide dependent protein kinase 1 PEBP1 Phosphatidylethanolamine binding protein 1 PIK3 Catalytic Subunits...RASA1 RASA2 RASA3 RAS p21 protein activator RASAL RASAL1 RASAL2 RASAL3 RAS protein activator like RASGRF ...

...fusion protein. Read more about epitope tags and protein tags . Tag or Fusion Protein Common...Return to top Epitope Tag or Fusion Protein Tags and fusion proteins are excellent tools for further ...Another common scenario is fusing your protein to another protein, such as GFP, which allows you to...localization of your protein. Additionally, tags are commonly added to aid in protein purification. Just...production of His6-MBP-tagged fusion proteins in E. coli SUMO Protein purification pCIOX - Bacterial expression...tagged recombinant protein pDest-Sumo - For prokaryotic expression of target protein as N-terminal fusion...two sgRNAs from an hU6 and an mU6 promoter Protein-protein interaction Proximity-dependent biotin identification...

...ll express your gRNA and Cas9 protein, which will form a ribonucleoprotein complex through interactions...naturally-occurring Acr (Anti-CRISPR) proteins can help control CRISPR activity. Acr proteins have evolved in phages ..., or inducing degradation of the Cas9 protein. Most Acr proteins are specific to a particular type of ...and antibody-activator effector proteins; long-term imaging of proteins in living cells (Figure 9B) dCas9...with different fluorescent proteins or by fusing the gRNAs to orthogonal protein-interacting RNA aptamers...edition) CRISPR software and depositor protocols Video protocol for genomic deletions in mammalian cell...applicability of base editing through protein engineering and protein delivery. Nature Communications . 8...

...truncated protein. Targeting an exon coding for an essential protein domain could abolish protein activity...review the protocol associated with the specific plasmid in question (see CRISPR protocols from Addgene...modified mouse lines. Protein delivery Lastly, you can deliver Cas-gRNA ribonucleoprotein (RNP) complexes directly...to translate mRNA to protein. Instead, you directly combine purified Cas protein and in vitro transcribed...Cationic lipid-mediated delivery of proteins enables efficient protein-based genome editing in vitro and...our CRISPR Guide . There are many different Cas proteins available, and researchers can adapt these experiments...a DNA donor template Cas9 or Cas9 fusions with proteins that promote HDR Requires a repair template Lower...

...expression analysis and protein fusion vectors for detecting and tracking proteins using tags such as NanoLuc...system is ideal for protein:protein interaction (PPI) studies, where each protein of interest is fused...useful for detecting and tracking key drug target proteins. Depositor...example, search by response element, reporter, protein, or tag, such as "NFAT", "luciferase", "Ras", "...pathways, investigating RNA regulation, tracking protein degradation, analyzing CRISPR-mediated gene editing...where you want minimal disruption of endogenous protein expression and interactions, such as exploring ...the virus of interest and monitoring endogenous protein dynamics in highly scalable assays. HaloTag Fusions...

... CRISPR Protein Tagging CRISPR Plasmids - Protein Tagging Browse CRISPR Plasmids...provided their protocol for homology arm cloning: Mendenhall & Myers FETCh-seq Protocol 134.7 KB Mendenhall...Xenopus CRISPR Resources CRISPR Guide Viral Preps Protocols gRNA Design Tools CRISPR Blog Posts The CRISPR...CRISPR system has been adapted to allow tagging of proteins expressed from their natural chromosomal context...tagging techniques, as well as the plasmids and protocols needed for using them, are described below. Read...add a tag (currently using FLAG) to endogenous proteins. This CRISPR/Cas plasmid-system consists of two...design and clone in a gRNA(s) to guide the Cas9 protein to your target sequence, as well as design and ...

...-producing proteins for detection of gene expression, protein localization, protein–protein interactions...Fluorescent Protein Guide for more plasmids containing fluorescent proteins. A set of fluorescent protein-based...plants. ID Plasmid Description PI Kits for Protein–Protein Interactions The following plasmid kits contain...variety of reporter plasmids for the study of protein–protein interactions in plants. ID Kit Description ...2in1 Plasmid Toolkit Plasmids for analysis of protein-protein interactions in planta via ratiometric Bimolecular...complementation (MoBiFC) toolkit for analyzing protein-protein interactions in plants. Golden Gate (MoClo)...expressing sgRNA and Cas9 in rice . Includes a protocol (Link opens in a new window) for targeted gene...