We narrowed to 50 results for: prot

...Protein A or Protein G Recombinant Antibody Affinity Purification with Protein A or Protein G You may also...Antibodies Immunocytochemistry Protocol Western Blot Protocol Introduction This protocol describes how to affinity... using Protein A or Protein G columns. Protocols...antibodies from cell culture supernatant using Protein A or Protein G columns. Sharing speeds science. We believe...outcomes when using these protocols. However, please be aware that the protocol may need to be adjusted ..., Thermo Fisher 21028 Protein G GraviTrap columns, Cytiva 28-9852-55 or rProtein A GraviTrap columns, ...Transfection for Recombinant Antibody protocol ). Pro-Tip Add protease inhibitors to 1X if this has not already...

... Protocols Pipetting Pipetting Protocol Intro to the Lab Bench Check out more protocols and videos...negatively impact your experimental results. This protocol will guide you in selecting the correct pipette...different mechanisms to disperse liquids, this protocol is focused on single channel pipettes. These come...a multichannel pipette. Please watch Addgene’s protocol on multichannel pipetting to learn more....

...transformations. Last Update: Nov. 13, 2017 Protocol Video Watch the protocol video below to learn how to isolate... Protocols Bacterial Transformation Bacterial Transformation...bacteria time to generate the antibiotic resistance proteins encoded in the plasmid backbone so that they will...higher efficiency is needed follow the complete protocol. Thaw the competent cells in your hand instead...

...Single-Stranded DNA-Binding protein (ET SSB) to the isothermal reaction mix. ET SSB protects 3’ ssDNA ends from... Protocols Gibson Cloning Gibson Assembly Cloning You may also...Methods Empty Backbone Guide Molecular Biology Protocols Summary In 2009 Dr. Daniel Gibson and colleagues...within a single buffer mixture and an optional SSB protein to improve accuracy and efficiency. Each enzyme...

...Weighing Reagents Protocol Intro to the Lab Bench Check out more protocols and videos to help you get started...In this protocol you will learn the basics of how to weigh reagents. Protocols... Protocols Weighing Reagents Weighing...affect the outcome of your experiment. In this protocol, you will learn the basics of how to weigh reagents...reagents. Wear the appropriate personal protective equipment (PPE) prior to handling the materials. Equipment... Make sure that any spills produced during the protocol are cleaned up. Discard the weighing boat or weighing...

... Protocols Water Bath Water Bath Protocol Intro to the Lab Bench Check out more protocols and videos...between 4°C and room temperature. This general protocol suits almost any water bath. Video Watch the video...the appropriate temperature as described in your protocol. How to set the temperature will vary depending...

...plate. Last Update: Feb. 28, 2017 Protocol Video Watch the protocol video below to learn how to isolate... Protocols Streaking Bacteria on LB Agar Plate Streaking ...mixture of plasmids in your purified DNA. This protocol explains how to isolate a single bacterial colony...

...Protocols for plasmid cloning by PCR.... Protocols Plasmid Cloning by PCR Plasmid Cloning by PCR You may...

...-restriction site-your sequence). Protocol Video Watch the protocol video below to learn how to design... Protocols Designing Primers How to Design a Primer You may also like...

...In this protocol you will learn the basics of how to use a light microscope.... Protocols Using a Light ...Microscope Intro to the Lab Bench Check out more protocols and videos to help you get started in the lab!...microscopy and still have more to learn, so, for this protocol, we'll focus on one of the most common types that...Procedure Set up your microscope by removing any protective cover that may be on it and plugging in the power...

...website . Last Upload: May 18, 2018 Protocol Video Watch the protocol video below to learn how to analyze...Protocol for how to perform a diagnostic digest to verify plasmid size, verify insert orientation, and... Protocols Diagnostic Restriction Digest Diagnostic Restriction Digest You may also like... Restriction...should have the correct orientation. For detailed protocols as well as tips and tricks see the Restriction...

...lengths). Last Update: Feb. 20, 2018 Protocol Video Watch the protocol video below to learn how to perform...Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and... Protocols Agarose Gel Electrophoresis Agarose Gel Electrophoresis You may also...EtBr is a known mutagen. Wear a lab coat, eye protection and gloves when working with this chemical. Note... damage to the DNA. Note: When using UV light, protect your skin by wearing safety goggles or a face shield...

...Protocol - How to Ligate Plasmid DNA... Protocols DNA Ligation DNA Ligation You may also like... Plasmid... to fuse the two ends of the vector together). Protocol: Standard Insert + Vector DNA Ligation Before ...

...following protocol is for inoculating an overnight culture of liquid LB with bacteria. Video Protocol Watch... the protocol video below to learn how to inoculate bacteria in liquid culture. Written Protocol Prepare...Protocol for Inoculating a Bacterial Culture Protocols... Protocols Inoculating a Liquid Bacterial Culture Inoculating...cloudy haze in the media (Figure 1). Notes: Some protocols require bacteria to be in the log phase of growth...growth. Check the instructions for your specific protocol and conduct an OD600 to measure the density of...culture by following the Isolating Your Plasmid DNA protocol. Figure 1: Media without growth (top) and with...

... Protocols pLKO.1 Protocol pLKO.1 - TRC Cloning Vector Addgene Plasmid 10878. Protocol Version ... RNAi Tools page. E.2 Protocol for Producing Lentiviral Particles This protocol is for transfection in... on your experiment Detailed protocols for preparing polybrene, protamine sulfate, and puromycin are located... stable at 4°C for several years. Protamine Sulfate Store protamine sulfate (MP Biomedicals catalog #194729...Addgene's protocol for using the pLKO.1 cloning vector, a backbone used by the RNAi consortium for targeting...Copyright Addgene 2006, All Rights Reserved. This protocol is provided for your convenience. See “warranty...Lentiviral Particles E.1 Recommended materials E.2 Protocol for producing lentiviral particles F. Infecting...

... Protocols Sequence Analysis Sequence Analysis of a Plasmid...plasmid features (such as the gene/insert, fusion proteins, point mutations, deletions, etc.) involves selecting...

...concentration. Last Update: Oct. 27, 2017 Protocol Video Watch the protocol video below to learn how to spread... Protocols Streaking Bacteria on LB Agar Plate Over-Agar ...Plasmid DNA from Bacterial Culture Introduction This protocol describes methodology for plating antibiotic over-agar...separate batches of antibiotic-containing agar. This protocol will focus specifically on selection with carbenicillin...transformation page for a detailed heatshock transformation protocol. Plate 50 µL of transformed E. coli /rescue media...

... Virus Blog Posts Mol Bio Protocols Viral Service Introduction This protocol can be used to generate stable...Protocol to generate stable cell lines expressing a gene of interest from an integrated lentiviral vector... Protocols Generating Stable Cell Lines with Lentivirus Generating Stable Cell Lines with Lentivirus...integrated lentiviral vector. Unlike the short-term protein expression observed using transient transfection...lines using lentiviral vectors enables long-term protein expression studies. Moreover, repeating experiments...lentiviral vectors deliver antibiotic resistance. This protocol was established using 293T cells but can be adapted...FBS lot numbers to find one suitable with your protocols. FBS can be purchased already heat-inactivated...

...stored stably at -80°C for many years. Protocol Video Watch the protocol video below to learn how to create...Protocol for creating a glycerol stock, including additional tips for long-term storage. Protocols... Protocols Creating...

... Protocols Plasmid Modification by Annealed Oligo Cloning...using a commercially available kit or standard protocol. Anneal oligos The oligos should be resuspended...