...desired bacterial strain and you will not need to obtain more competent cells and retransform. Bacteria on ... of Plasmids You may also like... Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture...the following day. Tips and FAQ The optimal concentration of long-term glycerol storage is unknown. Most...

...Digest of Plasmid DNA DNA Ligation Bacterial Transformation Summary Oligo overlap cloning can be used anytime...50mM NaCL, 1mM EDTA) and mixed in equimolar concentrations. We recommend mixing 2μg each in a total volume...with 45μL nuclease-free water and quantify the concentration (should be about 8ng/μl). Mix the annealed oligos...the vector with 0.75-6 ng of annealed oligos). Transform 2-3μL into your favorite competent bacteria and...

...complementary to template strand). However, primers do not need to correspond to the template strand completely; it...primer corresponds completely to the template DNA strand so elongation can proceed. Usually a guanine or...

...These items should remain in the laboratory. When traveling between lab spaces, take your glove off of one... Consider nitrile gloves as an alternative. For extra precautions, tuck your sleeves into your gloves ...adhered to. None of the authors, contributors, administrators, or anyone else associated with Addgene, can...

...experimental procedures, such as minipreps and RNA extractions. Plus, centrifuges are useful for simply collecting...cousins, but can hold much larger containers. Ultracentrifuges are also generally floor sized centrifuges...you may need to add a blank. Blanks are often an extra tube filled with water to the same volume as your...

...Plasmids grown in Dam or Dcm methylase positive strains will be resistant to cleavage at certain restriction...variety of reasons, including high glycerol concentration. Learn more at (Link opens in a new window) ...

...also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture...

...digest. Although this is never an ideal cloning strategy, sometimes it cannot be avoided. If you do have...