Lentivirus ddPCR Titration
Type
Protocol
...5 mL glutaGRO 50 U/mL benzonase: 15 mL DMEM Complete 3 µL of 250 U/µL benzonase Procedure Transducing ...Seed and transduce cells Day 4: Treat cells with Benzonase and harvest cells Day 4+: ddPCR and analysis Equipment...Thermo Fisher, 12605010) Ethanol, VWR, EX0276-1 Benzonase 250 U/µl, Millipore #71205-3 Polybrene 10 mg/mL...concentration is 10 µg/mL. Mix well and incubate 72 h. Benzonase Digestion and Cell Harvest Gently aspirate media...conditions with others. Gently add 1.5 mL of 50 U/mL Benzonase in DMEM complete to each well. Incubate at 37 ...