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We narrowed to 3 results for: Benzonase

Showing: 1 - 3 of 3 results
  1. Lentivirus ddPCR Titration

    Type
    Protocol
    ...5 mL glutaGRO 50 U/mL benzonase: 15 mL DMEM Complete 3 µL of 250 U/µL benzonase Procedure Transducing ...Seed and transduce cells Day 4: Treat cells with Benzonase and harvest cells Day 4+: ddPCR and analysis Equipment...Thermo Fisher, 12605010) Ethanol, VWR, EX0276-1 Benzonase 250 U/µl, Millipore #71205-3 Polybrene 10 mg/mL...concentration is 10 µg/mL. Mix well and incubate 72 h. Benzonase Digestion and Cell Harvest Gently aspirate media...conditions with others. Gently add 1.5 mL of 50 U/mL Benzonase in DMEM complete to each well. Incubate at 37 ...
  2. AAV Production in HEK293 Cells

    Type
    Protocol
    ...units of benzonase per mL of viral suspension (you should have ~18 mL from steps 12 & 13). Benzonase is an...expressing your gene of interest Triton X-100 Benzonase/DNAse I (Millipore 71205-3) 40% Polyethylene Glycol...
Showing: 1 - 3 of 3 results