...cut your plasmid into specific sized pieces and analyze the resulting fragments by gel electrophoresis ...DNA analysis tools, including Addgene’s Sequence Analyzer , allow you to identify which restriction sites...Watch the protocol video below to learn how to analyze a restriction digest. Equipment Electrophoresis...

...entire first gel lane (as in Figure 2). Select Analyze . Select Gels . Select Select First Lane . Drag...Drag the box to the next lane. Select Analyze . Select Gels . Select Select Next Lane . Repeat for all lanes... the protein bands as follows: Figure 3 Select Analyze . Select Gels . Select Plot Lanes . One graph per...

...Plasmid Cloning Protocols for constructing and analyzing your plasmids. Virus Protocols for titering and... spectrophotometer Restriction Digests Cut and analyze plasmid DNA with restriction enzymes Agarose Gel...

...Protocol Video Watch the video below to learn how to analyze your restriction digest results. Equipment Electrophoresis...sequence analysis program such as Addgene's Sequence Analyzer . Determine an appropriate reaction buffer by ...

...vector into a new vector that is better suited for analyzing the gene’s function. The process is shown graphically... DNA analysis tool, such as Addgene’s Sequence Analyzer , to allow you to identify which restriction sites...

...spectrophotometer (such as a Nanodrop), agarose gel, or bioanalyzer. For more information on nucleic acid quantification...

...bring your materials to a particular temperature, catalyze chemical reactions such as restriction digests...

...performed by the T4 DNA ligase enzyme. The DNA ligase catalyzes the formation of covalent phosphodiester linkages...

...goggles or a face shield, gloves and a lab coat. Analyzing Your Gel: Using the DNA ladder in the first lane...

...DNA analysis tools, including Addgene’s Sequence Analyzer , allow you to identify which restriction sites...

...Monitor cells for growth and expand cells Day 14–30: Analyze and expand monoclonal lines of interest Equipment...

...virus in reaction mix (4 µL) Sample Data When analyzing data there should be a clear distinction between...

...primers/probe mixes prior to use. Sample Data When analyzing data there should be a clear distinction between...