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Addgene

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We narrowed to 3 results for: crispr cas9 plasmids

Showing: 1 - 3 of 3 results

  1. Video Library

    Type
    Protocol
    ...mammalian cells lines using CRISPR/Cas9 Genomic Deletions with CRISPR Protocol Over-Agar Antibiotic Plating...Storing and Handling Addgene Plasmids What to do after you receive your plasmids from Addgene Instructions...Instructions for Storing and Handling Plasmids Genomic Deletions with CRISPR Generate genomic deletions in mammalian...Protocol Diagnostic Restriction Digest Verify a plasmid using restriction enzymes Diagnostic Restriction...Transformations We walk you through a standard plasmid transformation protocol and offer some tips and...Center How to Deposit Your guide to depositing plasmids with Addgene Depositing Page Career Videos (Link...

  2. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...Addgene plasmid #52962 ) and is described in Improved vectors and genome-wide libraries for CRISPR screening...A549 cells stably expressing Cas9. A549 cells were transduced with lentiCas9-Blast 1 and then selected with...Figure 2: Cas9 expression in monoclonal cell lines generated from A549 cells transduced with lentiCas9-Blast... express Cas9 and are included as a control (WT). Vinculin is a loading control. 1 lentiCas9-Blast was...additional 3 weeks under blasticidin selection. Anti-Cas9 Western blotting was performed on whole-cell extracts...

  3. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...A549 cells stably expressing Cas9. A549 cells were transduced with lentiCas9-Blast and then selected with...single cells for 13 days. lentiCas9-Blast was a gift from Feng Zhang (Addgene plasmid #52962 ) and is described... Improved vectors and genome-wide libraries for CRISPR screening. Sanjana NE, Shalem O, Zhang F. Nature...
Showing: 1 - 3 of 3 results