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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 6 results for: human dat

Showing: 1 - 6 of 6 results

  1. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...concentration!). Sample Data Figure 1: The plate was coated with serial dilutions of human recombinant purified...D7]. Plates were incubated with HRP-linked anti-Human IgG secondary antibody for 2 hours, reacted with... Protocols Antibody Validation Using the Indirect ELISA Method Antibody Validation Using the Indirect ...ImmunoSorbent Assay (ELISA) against a purified antigen to validate an antibody. This protocol will describe how to...that the protocol may need to be adjusted to accommodate slight differences between products. Addgene ...

  2. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...backbone used by the RNAi consortium for targeting human and mouse genes....built a library of shRNAs directed against 15,000 human and 15,000 mouse genes. Addgene is working with ...www.addgene.org/10878/ . Description Vector Element U6 Human U6 promoter drives RNA Polymerase III transcription...preintegration complex in the transduced cells. hPGK Human phosphoglycerate kinase promoter drives expression...209-216. PubMed . A lentiviral RNAi library for human and mouse genes applied to an arrayed viral high-content...Institute's TRC shRNA Design Process . To minimize degradation of off-target mRNAs, use NCBI’s BLAST program...days. h. Assay infected cells. The following recommendations are guidelines for the number of days you should...

  3. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...with microbes that don’t cause disease in healthy humans, for example, non-pathogenic E . coli . BSL-2 is...with chemicals, first review their material safety data sheets (MSDS). While You Work Dispose of sharps ...

  5. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...Miller Felix D., Rist Wolfgang, and Lamla Thorsten. Human Gene Therapy Methods. August 2015, 26(4): 147-157...aliquot and store at -80 °C for long term. Sample Data Figure 1: Iodixanol gradient before ultracentrifugation...

  6. Lentivirus ddPCR Titration

    Type
    Protocol
    ...aatttctctgtcccactccatc PrimePCR ddPCR Copy Number Assay: RPP30, Human, Bio-Rad, 10031244 Reagent Preparation DMEM Complete...primers/probe mixes prior to use. Sample Data When analyzing data there should be a clear distinction between...sample data, RRE positive (blue) and negative (black) droplets Figure 2: ddPCR Lentivirus sample data, RPP30... your institution’s biosafety regulations. Last Update: July 7, 2023 Workflow Timeline Day 1: Seed and...When the droplet reading is complete, export the data from all wells as a CSV file which will be used ...
Showing: 1 - 6 of 6 results