... to Top A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium The pLKO.1 cloning vector is the backbone...use of this vector. A.2 Map of pLKO.1 pLKO.1 is a replication-incompetent lentiviral vector chosen by the...pLKO.1 TRC-cloning vector. Addgene Plasmid Description pLKO.1 – TRC control Negative control vector containing...into the pLKO.1 vector, producing a final plasmid that expresses the shRNA of interest. C.1 Recommended ...pLKO.1 TRC Cloning Vector The following steps were the prevailing guidelines when the pLKO.1 vector was...digests. Digest pLKO.1 TRC-cloning vector with AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep...of Contents A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids...

...Volume of 1 mg/mL PEI (μL) 1:1 18.9 18.9 1:2 18.9 37.8 1:3 18.9 56.7 1:4 18.9 75.6 1:5 18.9 94.5 1:6 18.9...protein expression. Sample Data Legend: Lenti-X 293T cells were transfected using 1:1, 1:2, 1:3 and 1:6 µg.... Thawed aliquots should be discarded after 1–2 months. 1 mg/mL polyethylenimine, linear MW 25,000 Da ...stable. Dilute 1:3 (µg DNA:µg PEI) in 500 µL total of OptiPro SFM (per 10 cm plate). 56.7 µL of 1 mg/mL PEI...µg of pRosetta :µg of PEI. The 1:2 and 1:3 ratios provided high transfection efficiencies as can be seen...General Transfection You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral...polyethylenimine. Transfections allow for transient expression of a gene of interest in a target cell line and...