...digests. Digest pLKO.1 TRC-cloning vector with AgeI. Mix: 6 μg pLKO.1 TRC-cloning vector (maxiprep or miniprep...Back to Top A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium The pLKO.1 cloning vector is the backbone...Appendix I.1. Sequence of pLKO.1 TRC-Cloning Vector Click here to see the sequence of pLKO.1 TRC-cloning vector...conjunction with the pLKO.1 TRC-cloning vector. Addgene Plasmid Description pLKO.1 – TRC control Negative ...GTATAGCTTAACGTAGGCATT 3’ Back to Top C. Cloning Oligos into pLKO.1 The pLKO.1-TRC cloning vector contains a 1.9kb stuffer...Table of Contents A. pLKO.1-TRC Cloning Vector A.1 The RNAi Consortium A.2 Map of pLKO.1 A.3 Related plasmids... Protocols pLKO.1 Protocol pLKO.1 - TRC Cloning Vector Addgene Plasmid 10878. Protocol...

...Independent Cloning (LIC) Scarless cloning with Type II restriction enzymes and T4 polymerase pLKO.1 - TRC Cloning...Cloning Vector Cloning protocols for using the pLKO.1 vector, a backbone used by the RNAi consortium for...Plasmid Cloning Name Description (Link opens in a new window) Link to Video Restriction Cloning Assemble...ligation Cloning by PCR Generate restriction sites by PCR Modification by Annealed Oligo Cloning Add a short...(PPE) for BSL-1 and BSL-2 Labs Learn how to best protect yourself when working in BSL-1 and BSL-2 labs...Levels One and Two (BSL-1 and BSL-2) Safety measures for laboratories operating at BSL-1 and BSL-2 Watch the...can be used for basic molecular biology, plasmid cloning, and titering and testing your viral preparations...