Western Blot
Type
Protocol
...transfer stack. Set the Top Stack to one side and discard the white separator. Keep the Bottom Stack in the...Prestained protein ladder Ethanol iBlot 2 PVDF Mini Stack, Thermo Fisher IB24002 20X TBS Tween-20 Nonfat milk...the plastic tray. Place the Bottom Stack on the blotting surface. Align the electrical contacts on the...place it on the transfer membrane of the bottom stack. Soak a piece of iBlot Filter Paper in deionized... the air bubbles with the roller. Place the Top Stack over the pre-soaked filter paper. Remove air bubbles...roller. Place the Absorbent Pad on top of the Top Stack such that the electrical contacts are aligned with...