Welcome to Vector Database!
Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
- Plasmid Type
- Cloning Method
- This was also constructed from the SfiI fragment of EBOpSV2neo (hygromycin phosphotransferase gene, oriP, and EBNA-1 gene).  This is a shuttle vector permitting expression in primate cell lines. It is useful for subcloning.  This was constructed from the HindIII/EcoRI fragment of pcDV (pBR322 ori, beta-lactamase, SV40 late polyadenylation signal) and the HindIII/PstI fragment of pL (SV40 early promoter and SV40 late intervening sequence).  Restriction digests of the clone give the following sizes (kb): HindIII/EcoRI--7.0, 3.5, 0.6; EcoRI--7.0, 4.0; SalI--10.6; XbaI--10.6. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli, human cell line, simian cell line, dog cell line. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number