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Addgene

Vector Database


Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: EBOpLPP

Information

Source/Vendor
ATCC
Plasmid Type
Unspecified
Cloning Method
Unknown
Size
10572
Notes
This was also constructed from the SfiI fragment of EBOpSV2neo (hygromycin phosphotransferase gene, oriP, and EBNA-1 gene). [1] This is a shuttle vector permitting expression in primate cell lines. It is useful for subcloning. [2] This was constructed from the HindIII/EcoRI fragment of pcDV (pBR322 ori, beta-lactamase, SV40 late polyadenylation signal) and the HindIII/PstI fragment of pL (SV40 early promoter and SV40 late intervening sequence). [1] Restriction digests of the clone give the following sizes (kb): HindIII/EcoRI--7.0, 3.5, 0.6; EcoRI--7.0, 4.0; SalI--10.6; XbaI--10.6. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli, human cell line, simian cell line, dog cell line. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
Catalog Number
37704
Stable
Unspecified
Constitutive
Unspecified
Viral/Non-Viral
Unspecified