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Vector Database

Welcome to Vector Database!

Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.

This vector is NOT available from Addgene and the database is no longer actively maintained.

This vector is not available from Addgene.

Plasmid: lambda DL11


Plasmid Type
Cloning Method
A BamHI digest removes a 930 bp fragment containing the lacZalpha coding frame. Thus use of BamHI as a cloning site and selection of clear plaques on Xgal media may not indicate the presence of a recombinant insert. lambdaDL10 (ATCC 37489) and lambdaDL11 (ATCC 37490) have opposite orientations of the restriction sites within lacZ. A 435 bp HaeII fragment of pUC9, encoding beta-galactosidase, was inserted into the HindIII site of lambdaD69 to produce lambdaDL9. The multiple cloning sites of M13mp11 were crossed into lambdaDL9 to produce lambdaDL11. E.coli JM109 is not a good host for this vector. (personal communication) If HindIII, SacI, XbaI, XhoI, or BamHI are used as cloning sites, the vector can accept fragments of 0 - 12 kb. Inserts in HindIII, SacI, XbaI, BamHI, and SalI inactivate lacZ. If SalI is used as a cloning site, the vector can accept fragments of 9 - 20 kb. Replacement of the SalI fragment with a DNA insert results in a lambdacIII- phage that forms virtually clear plaques. Medium is 1227 LB plus ampicillin. Hosts: bacteria-free lysate, E.coli C600, E.coli JM101. (Information source: VectorDB ( ).)
Catalog Number