Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
Plasmid: pCMVbeta
Information
- Source/Vendor
- ATCC, Clontech
- Alt Name
- pCMV-beta-gal, pCMV-LacZ
- Plasmid Type
- Mammalian Expression
- Promoter
- CMV
- Cloning Method
- Unknown
- Size
- 7164
- 5' Sequencing 1 Primer
- CMV-F
- 3' Sequencing 1 Primer
- EBV-rev
- Bacterial Resistance
- Ampicillin
- Notes
- ATCC size is 7300 bp. The order of the major features in this plasmid is [1]: pUC19 - EcoRI - CMV immediate early promoter/enhancer - SV40 late 16S/19S splice signals -NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI - HindIII - pUC19. The lacZ gene may be excised by NotI and replaced with alternative sequences. [1] Designed to express high levels of beta-galactosidase or an alternative gene product. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors. [1] Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining. [1] Restriction digests of the clone give the following sizes (kb): EcoRI--7.3; HindIII--7.3; EcoRI/HindIII--4.6, 2.7; NotI--3.8, 3.5; BamHI--3.65; 3.45; 0.2. (ATCC staff) Medium is -1 TB plus ampicillin (200 ug/ml). Hosts: E.coli DH5alpha, monkey COS cells, vertebrate cells, E.coli. Related vectors: pADbeta, pNASSbeta, pSVbeta, pTKbeta. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- ATCC: 77177; Clontech: PT2004-5
- GenBank
- U02451
- Stable
- Transient
- Constitutive
- Constitutive
- Viral/Non-Viral
- Nonviral