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Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
|Alt Name:||pCMV-beta-gal, pCMV-LacZ|
|Plasmid Type:||Mammalian Expression|
|5' Sequencing 1 Primer:||CMV-F|
|3' Sequencing 1 Primer:||EBV-rev|
ATCC size is 7300 bp. The order of the major features in this plasmid is : pUC19 - EcoRI - CMV immediate early promoter/enhancer - SV40 late 16S/19S splice signals -NotI - lacZ - NotI - SV40 late polyadenylation signal - XbaI - HindIII - pUC19. The lacZ gene may be excised by NotI and replaced with alternative sequences.  Designed to express high levels of beta-galactosidase or an alternative gene product. May be used as a reference plasmid in transfection experiments or to monitor the effect of trans-acting factors.  Reporter plasmid permitting visual detection of beta-galactosidase activity by histochemical staining.  Restriction digests of the clone give the following sizes (kb): EcoRI--7.3; HindIII--7.3; EcoRI/HindIII--4.6, 2.7; NotI--3.8, 3.5; BamHI--3.65; 3.45; 0.2. (ATCC staff) Medium is -1 TB plus ampicillin (200 ug/ml). Hosts: E.coli DH5alpha, monkey COS cells, vertebrate cells, E.coli. Related vectors: pADbeta, pNASSbeta, pSVbeta, pTKbeta. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
|Catalog Number:||ATCC: 77177; Clontech: PT2004-5|