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Vector Database

Welcome to Vector Database!

Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.

This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.

This vector is NOT available from Addgene.

Plasmid: pCDFDuet-1

Source/Vendor: Novagen (EMD Millipore)
Alt Name: pCDFDuet™-1
Analyze: Sequence
Plasmid Type: Bacterial Expression
Promotor: AmpR
Expression Level: Unknown
Cloning Method: Restriction Enzyme
Size: 3781
5' Sequencing 1 Primer: ACYCDuetUP1
5' Sequencing 1 Primer Sequence: GGATCTCGACGCTCTCCCT
3' Sequencing 1 Primer: DuetDOWN1
3' Sequencing 1 Primer Sequence: GATTATGCGGCCGTGTACAA
5' Sequencing 2 Primer: DuetUP2
5' Sequencing 2 Primer Sequence: TTGTACACGGCCGCATAATC
3' Sequencing 2 Primer: T7term
3' Sequencing 2 Primer Sequence: GCTAGTTATTGCTCAGCGG
5' Terminal: N-Term
5' Terminal 2: N-Term
3' Terminal: C-Term
3' Terminal 2: C-Term
Tag 1: 6xHis
Tag 2: S15
Bacterial Resistance: Streptomycin
pCDFDuet™-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. This vector can be transformed into the same cell with pETDuet™-1, pACYCDuet™-1, and pRSFDuet™-1 or pCOLADuet™-1 for the coexpression of up to eight target genes. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.
Stable: Unspecified
Constitutive: Unspecified
Viral/Non-Viral: Nonviral

Generated Plasmid Map