Welcome to Vector Database!
Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene.
This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details.
This vector is NOT available from Addgene.
|Source/Vendor:||Novagen (EMD Millipore)|
|Plasmid Type:||Bacterial Expression|
|Cloning Method:||Restriction Enzyme|
|5' Sequencing 1 Primer:||ACYCDuetUP1|
|5' Sequencing 1 Primer Sequence:||GGATCTCGACGCTCTCCCT|
|3' Sequencing 1 Primer:||DuetDOWN1|
|3' Sequencing 1 Primer Sequence:||GATTATGCGGCCGTGTACAA|
|5' Sequencing 2 Primer:||DuetUP2|
|5' Sequencing 2 Primer Sequence:||TTGTACACGGCCGCATAATC|
|3' Sequencing 2 Primer:||T7term|
|3' Sequencing 2 Primer Sequence:||GCTAGTTATTGCTCAGCGG|
|5' Terminal 2:||N-Term|
|3' Terminal 2:||C-Term|
pCDFDuet™-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. This vector can be transformed into the same cell with pETDuet™-1, pACYCDuet™-1, and pRSFDuet™-1 or pCOLADuet™-1 for the coexpression of up to eight target genes. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.