Addgene - pMD2.G Plasmid Data

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Didier Trono Lab Plasmids

This is commonly
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pLKO.1 - TRC cloning vector

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Plasmid 12259: pMD2.G

Gene/insert name:		VSV G
Insert size (bp):		Unknown
Species of gene(s):		Other
Vector backbone:		pMD2.G (Search Vector Database)
Type of vector:		Mammalian expression,Envelope
Backbone size (bp):		5824
5' Sequencing primer:		CMV Fwd (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		High Copy
Grow in standard E. coli @ 37C:		Yes
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		DH5a
Principal Investigator:		Didier Trono
Terms and Licenses:		MTA

Comments: Envelope plasmid. Known to work with most Aebischer and Trono lab lentiviral vectors, as well as the pLKO system.

Please note that the full sequence for this plasmid is approximated and not fully verified. Please visit the Trono lab (http://tronolab.epfl.ch) for cloning strategies, protocols, publications, and more. See LentiWeb (http://www.lentiweb.com) for discussion on cloning strategies and protocols.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Click on map to enlarge

Selected features

SV40pro_F_primer

59 - 40

CAG_enhancer

241 - 528

CMV_immearly_promoter

162 - 738

CMV_fwd_primer

695 - 715

CMV_promoter

696 - 765

pCEP_fwd_primer

739 - 758

LNCX_primer

741 - 765

CMV2_promoter

708 - 827

bGlob_int

1015 - 1398

vsv-G

1450 - 2973

Orf frame 1

1450 - 2985

pBR322_origin

4674 - 4055

Orf frame 2

5689 - 4829

Ampicillin

5689 - 4829

AmpR_promoter

5759 - 5731

Unique restriction sites

ApaI		18
HindIII		835
PmlI		1405
ClaI		1429
PstI		2238
AgeI		2431
BclI		2534
BstBI		2711
MscI		3726
NotI		3881
EagI		3881
FspI		5124

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 12259" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.