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pGL3-U6-sgRNA-PGK-puromycin Citations (38)

Plasmid Article: Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects.
Shen B, Zhang W, Zhang J, Zhou J, Wang J, Chen L, Wang L, Hodgkins A, Iyer V, Huang X, Skarnes WC Nat Methods. 2014 Mar 2. doi: 10.1038/nmeth.2857.
PubMed Journal

Articles Citing pGL3-U6-sgRNA-PGK-puromycin

Pseudovirus-based neuraminidase inhibition assays reveal potential H5N1 drug-resistant mutations. Lu Y, Jiang T. Protein Cell. 2013 May;4(5):356-63. doi: 10.1007/s13238-013-2125-y. Epub 2013 Apr 15. PubMed
Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. Niu Y, Shen B, Cui Y, Chen Y, Wang J, Wang L, Kang Y, Zhao X, Si W, Li W, Xiang AP, Zhou J, Guo X, Bi Y, Si C, Hu B, Dong G, Wang H, Zhou Z, Li T, Tan T, Pu X, Wang F, Ji S, Zhou Q, Huang X, Ji W, Sha J. Cell. 2014 Feb 13;156(4):836-43. doi: 10.1016/j.cell.2014.01.027. Epub 2014 Jan 30. PubMed
sgRNAcas9: a software package for designing CRISPR sgRNA and evaluating potential off-target cleavage sites. Xie S, Shen B, Zhang C, Huang X, Zhang Y. PLoS One. 2014 Jun 23;9(6):e100448. doi: 10.1371/journal.pone.0100448. eCollection 2014. PubMed
An efficient genotyping method for genome-modified animals and human cells generated with CRISPR/Cas9 system. Zhu X, Xu Y, Yu S, Lu L, Ding M, Cheng J, Song G, Gao X, Yao L, Fan D, Meng S, Zhang X, Hu S, Tian Y. Sci Rep. 2014 Sep 19;4:6420. doi: 10.1038/srep06420. PubMed
Dkk3 prevents familial dilated cardiomyopathy development through Wnt pathway. Lu D, Bao D, Dong W, Liu N, Zhang X, Gao S, Ge W, Gao X, Zhang L. Lab Invest. 2016 Feb;96(2):239-48. doi: 10.1038/labinvest.2015.145. Epub 2015 Dec 7. PubMed
GPI Anchor Modification Machinery Deficiency is Responsible for the Formation of Pro-PrP in BxPC-3 and Increases Cancer Cell Motility. Yang L, Gao Z, Hu L, Wu G, Yang X, Zhang L, Zhu Y, Wong BS, Xin W, Sy MS, Li C. J Biol Chem. 2015 Dec 18. pii: jbc.M115.705830. PubMed
CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients. Su S, Hu B, Shao J, Shen B, Du J, Du Y, Zhou J, Yu L, Zhang L, Chen F, Sha H, Cheng L, Meng F, Zou Z, Huang X, Liu B. Sci Rep. 2016 Jan 28;6:20070. doi: 10.1038/srep20070. PubMed
Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome. Wood LW, Cox NI, Phelps CA, Lai SC, Poddar A, Talbot C Jr, Mu D. Sci Rep. 2016 Feb 25;6:19857. doi: 10.1038/srep19857. PubMed

Associated Plasmids

CRISPR-Cas9 mediated genetic engineering for the purification of the endogenous integrator complex from mammalian cells. Baillat D, Russell WK, Wagner EJ. Protein Expr Purif. 2016 Dec;128:101-8. doi: 10.1016/j.pep.2016.08.011. Epub 2016 Aug 19. PubMed
MicroRNA-202 maintains spermatogonial stem cells by inhibiting cell cycle regulators and RNA binding proteins. Chen J, Cai T, Zheng C, Lin X, Wang G, Liao S, Wang X, Gan H, Zhang D, Hu X, Wang S, Li Z, Feng Y, Yang F, Han C. Nucleic Acids Res. 2016 Dec 19. pii: gkw1287. doi: 10.1093/nar/gkw1287. PubMed
CRISPR-Cas9-mediated disruption of PD-1 on human T cells for adoptive cellular therapies of EBV positive gastric cancer. Su S, Zou Z, Chen F, Ding N, Du J, Shao J, Li L, Fu Y, Hu B, Yang Y, Sha H, Meng F, Wei J, Huang X, Liu B. Oncoimmunology. 2016 Nov 22;6(1):e1249558. doi: 10.1080/2162402X.2016.1249558. eCollection 2017. PubMed
Isolated polycystic liver disease genes define effectors of polycystin-1 function. Besse W, Dong K, Choi J, Punia S, Fedeles SV, Choi M, Gallagher AR, Huang EB, Gulati A, Knight J, Mane S, Tahvanainen E, Tahvanainen P, Sanna-Cherchi S, Lifton RP, Watnick T, Pei YP, Torres VE, Somlo S. J Clin Invest. 2017 May 1;127(5):1772-1785. doi: 10.1172/JCI90129. Epub 2017 Apr 4. PubMed
CRISPR/Cas9-mediated ApoE-/- and LDLR-/- double gene knockout in pigs elevates serum LDL-C and TC levels. Huang L, Hua Z, Xiao H, Cheng Y, Xu K, Gao Q, Xia Y, Liu Y, Zhang X, Zheng X, Mu Y, Li K. Oncotarget. 2017 Jun 6;8(23):37751-37760. doi: 10.18632/oncotarget.17154. PubMed
RPL10L Is Required for Male Meiotic Division by Compensating for RPL10 during Meiotic Sex Chromosome Inactivation in Mice. Jiang L, Li T, Zhang X, Zhang B, Yu C, Li Y, Fan S, Jiang X, Khan T, Hao Q, Xu P, Nadano D, Huleihel M, Lunenfeld E, Wang PJ, Zhang Y, Shi Q. Curr Biol. 2017 May 22;27(10):1498-1505.e6. doi: 10.1016/j.cub.2017.04.017. Epub 2017 May 11. PubMed
Nuclear carbonic anhydrase 6B associates with PRMT5 to epigenetically promote IL-12 expression in innate response. Xu J, Xu X, Wang B, Ma Y, Zhang L, Xu H, Hu Y, Wu J, Cao X. Proc Natl Acad Sci U S A. 2017 Aug 8;114(32):8620-8625. doi: 10.1073/pnas.1700917114. Epub 2017 Jul 24. PubMed
Prion protein is required for tumor necrosis factor alpha (TNFalpha)-triggered nuclear factor kappaB (NF-kappaB) signaling and cytokine production. Wu GR, Mu TC, Gao ZX, Wang J, Sy MS, Li CY. J Biol Chem. 2017 Nov 17;292(46):18747-18759. doi: 10.1074/jbc.M117.787283. Epub 2017 Sep 12. PubMed
Engineered cells for costimulatory enhancement combined with IL-21 enhance the generation of PD-1-disrupted CTLs for adoptive immunotherapy. Shao J, Xu Q, Su S, Meng F, Zou Z, Chen F, Du J, Qian X, Liu B. Cell Immunol. 2017 Oct;320:38-45. doi: 10.1016/j.cellimm.2017.09.003. Epub 2017 Sep 7. PubMed
Multiplex Enhancer Interference Reveals Collaborative Control of Gene Regulation by Estrogen Receptor alpha-Bound Enhancers. Carleton JB, Berrett KC, Gertz J. Cell Syst. 2017 Oct 25;5(4):333-344.e5. doi: 10.1016/j.cels.2017.08.011. Epub 2017 Sep 27. PubMed

Associated Plasmids

Relationship between DNA Methylation in the 5' CpG Island of the SLC47A1 (Multidrug and Toxin Extrusion Protein MATE1) Gene and Interindividual Variability in MATE1 Expression in the Human Liver. Tanaka T, Hirota T, Ieiri I. Mol Pharmacol. 2018 Jan;93(1):1-7. doi: 10.1124/mol.117.109553. Epub 2017 Oct 25. PubMed
CRISPR-offinder: a CRISPR guide RNA design and off-target searching tool for user-defined protospacer adjacent motif. Zhao C, Zheng X, Qu W, Li G, Li X, Miao YL, Han X, Liu X, Li Z, Ma Y, Shao Q, Li H, Sun F, Xie S, Zhao S. Int J Biol Sci. 2017 Nov 1;13(12):1470-1478. doi: 10.7150/ijbs.21312. eCollection 2017. PubMed
Interindividual Differences in the Expression of ATP-Binding Cassette and Solute Carrier Family Transporters in Human Skin: DNA Methylation Regulates Transcriptional Activity of the Human ABCC3 Gene. Takechi T, Hirota T, Sakai T, Maeda N, Kobayashi D, Ieiri I. Drug Metab Dispos. 2018 May;46(5):628-635. doi: 10.1124/dmd.117.079061. Epub 2018 Feb 2. PubMed
Generation of GHR-modified pigs as Laron syndrome models via a dual-sgRNAs/Cas9 system and somatic cell nuclear transfer. Yu H, Long W, Zhang X, Xu K, Guo J, Zhao H, Li H, Qing Y, Pan W, Jia B, Zhao HY, Huang X, Wei HJ. J Transl Med. 2018 Feb 27;16(1):41. doi: 10.1186/s12967-018-1409-7. PubMed
Base editing with a Cpf1-cytidine deaminase fusion. Li X, Wang Y, Liu Y, Yang B, Wang X, Wei J, Lu Z, Zhang Y, Wu J, Huang X, Yang L, Chen J. Nat Biotechnol. 2018 Mar 19. pii: nbt.4102. doi: 10.1038/nbt.4102. PubMed

Associated Plasmids

Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression. Han Y, Liu Q, Hou J, Gu Y, Zhang Y, Chen Z, Fan J, Zhou W, Qiu S, Zhang Y, Dong T, Li N, Jiang Z, Zhu H, Zhang Q, Ma Y, Zhang L, Wang Q, Yu Y, Li N, Cao X. Cell. 2018 Apr 19;173(3):634-648.e12. doi: 10.1016/j.cell.2018.02.061. Epub 2018 Mar 29. PubMed
Tumor-secreted Pros1 inhibits macrophage M1 polarization to reduce antitumor immune response. Ubil E, Caskey L, Holtzhausen A, Hunter D, Story C, Earp HS. J Clin Invest. 2018 Jun 1;128(6):2356-2369. doi: 10.1172/JCI97354. Epub 2018 Apr 30. PubMed
BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity. Jiang W, Feng S, Huang S, Yu W, Li G, Yang G, Liu Y, Zhang Y, Zhang L, Hou Y, Chen J, Chen J, Huang X. Cell Res. 2018 Jun 6. pii: 10.1038/s41422-018-0052-4. doi: 10.1038/s41422-018-0052-4. PubMed

Associated Plasmids

Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing. Liu Z, Lu Z, Yang G, Huang S, Li G, Feng S, Liu Y, Li J, Yu W, Zhang Y, Chen J, Sun Q, Huang X. Nat Commun. 2018 Jun 14;9(1):2338. doi: 10.1038/s41467-018-04768-7. PubMed

Associated Plasmids

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines. Carleton JB, Berrett KC, Gertz J. J Vis Exp. 2018 Jun 2;(136). doi: 10.3791/57883. PubMed
Lipase maturation factor 1 affects redox homeostasis in the endoplasmic reticulum. Roberts BS, Babilonia-Rosa MA, Broadwell LJ, Wu MJ, Neher SB. EMBO J. 2018 Oct 1;37(19). pii: embj.201797379. doi: 10.15252/embj.201797379. Epub 2018 Aug 1. PubMed
Efficient base editing in methylated regions with a human APOBEC3A-Cas9 fusion. Wang X, Li J, Wang Y, Yang B, Wei J, Wu J, Wang R, Huang X, Chen J, Yang L. Nat Biotechnol. 2018 Aug 20. pii: nbt.4198. doi: 10.1038/nbt.4198. PubMed
Correction of the Marfan Syndrome Pathogenic FBN1 Mutation by Base Editing in Human Cells and Heterozygous Embryos. Zeng Y, Li J, Li G, Huang S, Yu W, Zhang Y, Chen D, Chen J, Liu J, Huang X. Mol Ther. 2018 Nov 7;26(11):2631-2637. doi: 10.1016/j.ymthe.2018.08.007. Epub 2018 Aug 14. PubMed
Phosphorylation-Mediated IFN-gammaR2 Membrane Translocation Is Required to Activate Macrophage Innate Response. Xu X, Xu J, Wu J, Hu Y, Han Y, Gu Y, Zhao K, Zhang Q, Liu X, Liu J, Liu B, Cao X. Cell. 2018 Nov 15;175(5):1336-1351.e17. doi: 10.1016/j.cell.2018.09.011. Epub 2018 Oct 11. PubMed
Base-Editing-Mediated R17H Substitution in Histone H3 Reveals Methylation-Dependent Regulation of Yap Signaling and Early Mouse Embryo Development. Yang G, Zhou C, Wang R, Huang S, Wei Y, Yang X, Liu Y, Li J, Lu Z, Ying W, Li X, Jing N, Huang X, Yang H, Qiao Y. Cell Rep. 2019 Jan 8;26(2):302-312.e4. doi: 10.1016/j.celrep.2018.12.046. PubMed
The molecular logic of Nanog-induced self-renewal in mouse embryonic stem cells. Heurtier V, Owens N, Gonzalez I, Mueller F, Proux C, Mornico D, Clerc P, Dubois A, Navarro P. Nat Commun. 2019 Mar 7;10(1):1109. doi: 10.1038/s41467-019-09041-z. PubMed

Associated Plasmids

Cancer testis antigen 55 deficiency attenuates colitis-associated colorectal cancer by inhibiting NF-kappaB signaling. Zhao H, Pan WM, Zhang HH, Song Y, Chen J, Xiang Y, Gu B, Li SZ, Du RL, Zhang XD. Cell Death Dis. 2019 Apr 3;10(4):304. doi: 10.1038/s41419-019-1537-x. PubMed
MED12 exerts an emerging role in actin-mediated cytokinesis via LIMK2/cofilin pathway in NSCLC. Xu M, Wang F, Li G, Wang X, Fang X, Jin H, Chen Z, Zhang J, Fu L. Mol Cancer. 2019 May 9;18(1):93. doi: 10.1186/s12943-019-1020-4. PubMed
Modeling Steatohepatitis in Humans with Pluripotent Stem Cell-Derived Organoids. Ouchi R, Togo S, Kimura M, Shinozawa T, Koido M, Koike H, Thompson W, Karns RA, Mayhew CN, McGrath PS, McCauley HA, Zhang RR, Lewis K, Hakozaki S, Ferguson A, Saiki N, Yoneyama Y, Takeuchi I, Mabuchi Y, Akazawa C, Yoshikawa HY, Wells JM, Takebe T. Cell Metab. 2019 May 30. pii: S1550-4131(19)30247-5. doi: 10.1016/j.cmet.2019.05.007. PubMed
Efficient Generation of Pathogenic A-to-G Mutations in Human Tripronuclear Embryos via ABE-Mediated Base Editing. Li G, Liu X, Huang S, Zeng Y, Yang G, Lu Z, Zhang Y, Ma X, Wang L, Huang X, Liu J. Mol Ther Nucleic Acids. 2019 Jun 5;17:289-296. doi: 10.1016/j.omtn.2019.05.021. PubMed

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