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lenti MS2-P65-HSF1_Hygro Citations (42)

Plasmid Article: Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.
Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F Nature. 2014 Dec 10. doi: 10.1038/nature14136.
PubMed Journal

Articles Citing lenti MS2-P65-HSF1_Hygro

Articles
Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease. Dahlman JE, Abudayyeh OO, Joung J, Gootenberg JS, Zhang F, Konermann S. Nat Biotechnol. 2015 Oct 5;33(11):1159-1161. doi: 10.1038/nbt.3390. PubMed
CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust reactivation of the HIV-1 latent reservoirs. Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young WB, Khalili K, Hu W. Sci Rep. 2015 Nov 5;5:16277. doi: 10.1038/srep16277. PubMed
Potent and Targeted Activation of Latent HIV-1 Using the CRISPR/dCas9 Activator Complex. Saayman SM, Lazar DC, Scott TA, Hart JR, Takahashi M, Burnett JC, Planelles V, Morris KV, Weinberg MS. Mol Ther. 2015 Nov 19. doi: 10.1038/mt.2015.202. PubMed
CRISPR-mediated Activation of Latent HIV-1 Expression. Limsirichai P, Gaj T, Schaffer DV. Mol Ther. 2015 Nov 26. doi: 10.1038/mt.2015.213. PubMed
Specific induction of endogenous viral restriction factors using CRISPR/Cas-derived transcriptional activators. Bogerd HP, Kornepati AV, Marshall JB, Kennedy EM, Cullen BR. Proc Natl Acad Sci U S A. 2015 Dec 29;112(52):E7249-56. doi: 10.1073/pnas.1516305112. Epub 2015 Dec 14. PubMed
Posttranscriptional m(6)A Editing of HIV-1 mRNAs Enhances Viral Gene Expression. Kennedy EM, Bogerd HP, Kornepati AV, Kang D, Ghoshal D, Marshall JB, Poling BC, Tsai K, Gokhale NS, Horner SM, Cullen BR. Cell Host Microbe. 2016 May 11;19(5):675-85. doi: 10.1016/j.chom.2016.04.002. Epub 2016 Apr 21. PubMed
Comparison of Cas9 activators in multiple species. Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon N, Collins JJ, Church G. Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871. PubMed

Associated Plasmids

Regulation of PCGEM1 by p54/nrb in prostate cancer. Ho TT, Huang J, Zhou N, Zhang Z, Koirala P, Zhou X, Wu F, Ding X, Mo YY. Sci Rep. 2016 Sep 29;6:34529. doi: 10.1038/srep34529. PubMed
Genome-scale deletion screening of human long non-coding RNAs using a paired-guide RNA CRISPR-Cas9 library. Zhu S, Li W, Liu J, Chen CH, Liao Q, Xu P, Xu H, Xiao T, Cao Z, Peng J, Yuan P, Brown M, Liu XS, Wei W. Nat Biotechnol. 2016 Dec;34(12):1279-1286. doi: 10.1038/nbt.3715. Epub 2016 Oct 31. PubMed

Associated Plasmids

Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB, Bassik MC. Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. PubMed

Associated Plasmids

Generation of induced pluripotent stem cells (iPSCs) stably expressing CRISPR-based synergistic activation mediator (SAM). Xiong K, Zhou Y, Hyttel P, Bolund L, Freude KK, Luo Y. Stem Cell Res. 2016 Nov 17;17(3):665-669. doi: 10.1016/j.scr.2016.10.011. PubMed
Rewiring human cellular input-output using modular extracellular sensors. Schwarz KA, Daringer NM, Dolberg TB, Leonard JN. Nat Chem Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253. PubMed

Associated Plasmids

Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 Mar 23. PubMed

Associated Plasmids

The Effects of Voluntary Physical Exercise-Activated Neurotrophic Signaling in Rat Hippocampus on mRNA Levels of Downstream Signaling Molecules. Solvsten CAE, Daugaard TF, Luo Y, de Paoli F, Christensen JH, Nielsen AL. J Mol Neurosci. 2017 Jun;62(2):142-153. doi: 10.1007/s12031-017-0918-9. Epub 2017 Apr 24. PubMed
A Wnt-producing niche drives proliferative potential and progression in lung adenocarcinoma. Tammela T, Sanchez-Rivera FJ, Cetinbas NM, Wu K, Joshi NS, Helenius K, Park Y, Azimi R, Kerper NR, Wesselhoeft RA, Gu X, Schmidt L, Cornwall-Brady M, Yilmaz OH, Xue W, Katajisto P, Bhutkar A, Jacks T. Nature. 2017 May 18;545(7654):355-359. doi: 10.1038/nature22334. Epub 2017 May 10. PubMed
Quantitative proteomics identify Tenascin-C as a promoter of lung cancer progression and contributor to a signature prognostic of patient survival. Gocheva V, Naba A, Bhutkar A, Guardia T, Miller KM, Li CM, Dayton TL, Sanchez-Rivera FJ, Kim-Kiselak C, Jailkhani N, Winslow MM, Del Rosario A, Hynes RO, Jacks T. Proc Natl Acad Sci U S A. 2017 Jul 11;114(28):E5625-E5634. doi: 10.1073/pnas.1707054114. Epub 2017 Jun 26. PubMed
EINCR1 is an EGF inducible lincRNA overexpressed in lung adenocarcinomas. Nowicki-Osuch K, Li Y, Challinor M, Gerrard DT, Hanley NA, Sharrocks AD. PLoS One. 2017 Jul 21;12(7):e0181902. doi: 10.1371/journal.pone.0181902. eCollection 2017. PubMed
An all-in-one UniSam vector system for efficient gene activation. Fidanza A, Lopez-Yrigoyen M, Romano N, Jones R, Taylor AH, Forrester LM. Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.1038/s41598-017-06468-6. PubMed
Evaluating Synthetic Activation and Repression of Neuropsychiatric-Related Genes in hiPSC-Derived NPCs, Neurons, and Astrocytes. Ho SM, Hartley BJ, Flaherty E, Rajarajan P, Abdelaal R, Obiorah I, Barretto N, Muhammad H, Phatnani HP, Akbarian S, Brennand KJ. Stem Cell Reports. 2017 Aug 8;9(2):615-628. doi: 10.1016/j.stemcr.2017.06.012. Epub 2017 Jul 27. PubMed

Associated Plasmids

Genome-scale activation screen identifies a lncRNA locus regulating a gene neighbourhood. Joung J, Engreitz JM, Konermann S, Abudayyeh OO, Verdine VK, Aguet F, Gootenberg JS, Sanjana NE, Wright JB, Fulco CP, Tseng YY, Yoon CH, Boehm JS, Lander ES, Zhang F. Nature. 2017 Aug 11. doi: 10.1038/nature23451. PubMed
Optimized strategy for in vivo Cas9-activation in Drosophila. Ewen-Campen B, Yang-Zhou D, Fernandes VR, Gonzalez DP, Liu LP, Tao R, Ren X, Sun J, Hu Y, Zirin J, Mohr SE, Ni JQ, Perrimon N. Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):9409-9414. doi: 10.1073/pnas.1707635114. Epub 2017 Aug 14. PubMed
Specific Expression of Interferon-gamma Induced by Synergistic Activation Mediator-Derived Systems Activates Innate Immunity and Inhibits Tumorigenesis. Liu S, Yu X, Wang Q, Liu Z, Xiao Q, Hou P, Hu Y, Hou W, Yang Z, Guo D, Chen S. J Microbiol Biotechnol. 2017 Oct 28;27(10):1855-1866. doi: 10.4014/jmb.1705.05081. PubMed
One-Step piggyBac Transposon-Based CRISPR/Cas9 Activation of Multiple Genes. Li S, Zhang A, Xue H, Li D, Liu Y. Mol Ther Nucleic Acids. 2017 Sep 15;8:64-76. doi: 10.1016/j.omtn.2017.06.007. Epub 2017 Jun 15. PubMed

Associated Plasmids

Bidirectional manipulation of gene expression in adipocytes using CRISPRa and siRNA. Lundh M, Plucinska K, Isidor MS, Petersen PSS, Emanuelli B. Mol Metab. 2017 Oct;6(10):1313-1320. doi: 10.1016/j.molmet.2017.07.001. Epub 2017 Jul 8. PubMed
Multiscale 3D Genome Rewiring during Mouse Neural Development. Bonev B, Mendelson Cohen N, Szabo Q, Fritsch L, Papadopoulos GL, Lubling Y, Xu X, Lv X, Hugnot JP, Tanay A, Cavalli G. Cell. 2017 Oct 19;171(3):557-572.e24. doi: 10.1016/j.cell.2017.09.043. PubMed
Fusion guide RNAs for orthogonal gene manipulation with Cas9 and Cpf1. Kweon J, Jang AH, Kim DE, Yang JW, Yoon M, Rim Shin H, Kim JS, Kim Y. Nat Commun. 2017 Nov 23;8(1):1723. doi: 10.1038/s41467-017-01650-w. PubMed
Conserved Gene Microsynteny Unveils Functional Interaction Between Protein Disulfide Isomerase and Rho Guanine-Dissociation Inhibitor Families. Moretti AIS, Pavanelli JC, Nolasco P, Leisegang MS, Tanaka LY, Fernandes CG, Wosniak J Jr, Kajihara D, Dias MH, Fernandes DC, Jo H, Tran NV, Ebersberger I, Brandes RP, Bonatto D, Laurindo FRM. Sci Rep. 2017 Dec 8;7(1):17262. doi: 10.1038/s41598-017-16947-5. PubMed
In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation. Liao HK, Hatanaka F, Araoka T, Reddy P, Wu MZ, Sui Y, Yamauchi T, Sakurai M, O'Keefe DD, Nunez-Delicado E, Guillen P, Campistol JM, Wu CJ, Lu LF, Esteban CR, Izpisua Belmonte JC. Cell. 2017 Dec 14;171(7):1495-1507.e15. doi: 10.1016/j.cell.2017.10.025. Epub 2017 Dec 7. PubMed

Associated Plasmids

Multimode drug inducible CRISPR/Cas9 devices for transcriptional activation and genome editing. Lu J, Zhao C, Zhao Y, Zhang J, Zhang Y, Chen L, Han Q, Ying Y, Peng S, Ai R, Wang Y. Nucleic Acids Res. 2018 Mar 16;46(5):e25. doi: 10.1093/nar/gkx1222. PubMed

Associated Plasmids

Polycomb protein RING1A limits hematopoietic differentiation in myelodysplastic syndromes. Palau A, Garz AK, Diesch J, Zwick A, Malinverni R, Valero V, Lappin K, Casquero R, Lennartsson A, Zuber J, Navarro T, Mills KI, Gotze KS, Buschbeck M. Oncotarget. 2017 Dec 1;8(70):115002-115017. doi: 10.18632/oncotarget.22839. eCollection 2017 Dec 29. PubMed
RNA-dependent chromatin targeting of TET2 for endogenous retrovirus control in pluripotent stem cells. Guallar D, Bi X, Pardavila JA, Huang X, Saenz C, Shi X, Zhou H, Faiola F, Ding J, Haruehanroengra P, Yang F, Li D, Sanchez-Priego C, Saunders A, Pan F, Valdes VJ, Kelley K, Blanco MG, Chen L, Wang H, Sheng J, Xu M, Fidalgo M, Shen X, Wang J. Nat Genet. 2018 Mar;50(3):443-451. doi: 10.1038/s41588-018-0060-9. Epub 2018 Feb 26. PubMed
TCF21/POD-1, a Transcritional Regulator of SF-1/NR5A1, as a Potential Prognosis Marker in Adult and Pediatric Adrenocortical Tumors. Passaia BDS, Dias MH, Kremer JL, Antonini SRR, de Almeida MQ, Fragoso MCBV, Lotfi CFP. Front Endocrinol (Lausanne). 2018 Feb 22;9:38. doi: 10.3389/fendo.2018.00038. eCollection 2018. PubMed
Simple method for assembly of CRISPR synergistic activation mediator gRNA expression array. Vad-Nielsen J, Nielsen AL, Luo Y. J Biotechnol. 2018 May 20;274:54-57. doi: 10.1016/j.jbiotec.2018.03.018. Epub 2018 Mar 26. PubMed
Next-generation CRISPR/Cas9 transcriptional activation in Drosophila using flySAM. Jia Y, Xu RG, Ren X, Ewen-Campen B, Rajakumar R, Zirin J, Yang-Zhou D, Zhu R, Wang F, Mao D, Peng P, Qiao HH, Wang X, Liu LP, Xu B, Ji JY, Liu Q, Sun J, Perrimon N, Ni JQ. Proc Natl Acad Sci U S A. 2018 May 1;115(18):4719-4724. doi: 10.1073/pnas.1800677115. Epub 2018 Apr 16. PubMed
An Integrated Genome-wide CRISPRa Approach to Functionalize lncRNAs in Drug Resistance. Bester AC, Lee JD, Chavez A, Lee YR, Nachmani D, Vora S, Victor J, Sauvageau M, Monteleone E, Rinn JL, Provero P, Church GM, Clohessy JG, Pandolfi PP. Cell. 2018 Apr 19;173(3):649-664.e20. doi: 10.1016/j.cell.2018.03.052. PubMed
Human pluripotent reprogramming with CRISPR activators. Weltner J, Balboa D, Katayama S, Bespalov M, Krjutskov K, Jouhilahti EM, Trokovic R, Kere J, Otonkoski T. Nat Commun. 2018 Jul 6;9(1):2643. doi: 10.1038/s41467-018-05067-x. PubMed

Associated Plasmids

Comprehensive off-target analysis of dCas9-SAM-mediated HIV reactivation via long noncoding RNA and mRNA profiling. Zhang Y, Arango G, Li F, Xiao X, Putatunda R, Yu J, Yang XF, Wang H, Watson LT, Zhang L, Hu W. BMC Med Genomics. 2018 Sep 10;11(1):78. doi: 10.1186/s12920-018-0394-2. PubMed
Systematic genetic mapping of necroptosis identifies SLC39A7 as modulator of death receptor trafficking. Fauster A, Rebsamen M, Willmann KL, Cesar-Razquin A, Girardi E, Bigenzahn JW, Schischlik F, Scorzoni S, Bruckner M, Konecka J, Hormann K, Heinz LX, Boztug K, Superti-Furga G. Cell Death Differ. 2018 Sep 20. pii: 10.1038/s41418-018-0192-6. doi: 10.1038/s41418-018-0192-6. PubMed
Intrinsic Nucleotide Preference of Diversifying Base Editors Guides Antibody Ex Vivo Affinity Maturation. Liu LD, Huang M, Dai P, Liu T, Fan S, Cheng X, Zhao Y, Yeap LS, Meng FL. Cell Rep. 2018 Oct 23;25(4):884-892.e3. doi: 10.1016/j.celrep.2018.09.090. PubMed

Associated Plasmids

Genomic sequencing and editing revealed the GRM8 signaling pathway as potential therapeutic targets of squamous cell lung cancer. Zhang P, Kang B, Xie G, Li S, Gu Y, Shen Y, Zhao X, Ma Y, Li F, Si J, Wang J, Chen J, Yang H, Xu X, Yang Y. Cancer Lett. 2019 Feb 1;442:53-67. doi: 10.1016/j.canlet.2018.10.035. Epub 2018 Oct 27. PubMed
Pooled extracellular receptor-ligand interaction screening using CRISPR activation. Chong ZS, Ohnishi S, Yusa K, Wright GJ. Genome Biol. 2018 Nov 26;19(1):205. doi: 10.1186/s13059-018-1581-3. PubMed

Associated Plasmids

A Transcription Factor Addiction in Leukemia Imposed by the MLL Promoter Sequence. Lu B, Klingbeil O, Tarumoto Y, Somerville TDD, Huang YH, Wei Y, Wai DC, Low JKK, Milazzo JP, Wu XS, Cao Z, Yan X, Demerdash OE, Huang G, Mackay JP, Kinney JB, Shi J, Vakoc CR. Cancer Cell. 2018 Dec 10;34(6):970-981.e8. doi: 10.1016/j.ccell.2018.10.015. Epub 2018 Nov 29. PubMed

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